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June 23, 2019

Laboratory Techniques

Western blotting Protocol

Introduction The Western blot is a biological technique that allows for specific identification and characterization of proteins. In this technique, the proteins are assorted based on their molecular weight, and type, through gel electrophoresis. The results of the electrophoresis are then transferred to a polyvinylidene fluoride (PVDF) membrane producing a band for each protein. The membrane containing bands is then