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June 25, 2019

Nucleic Acid Quantification Protocols
Laboratory Techniques

Nucleic Acid Quantification Protocols

Introduction: Quantitative Method DNA and RNA quantifications are broadly utilized in biological and biomedical research. Over the last ten years, many technologies have been developed to enable automated and high-throughput analyses. We are going to learn the assays and different methods of how DNA and RNA quantifications are carried out. Introduction and Principle: SCHMIDT and THANNHAUSER DNA, RNA, and Protein
Filtration Chromatography Protocol
Laboratory Techniques

Quantitative Phosphate Protocol

Introduction: Determination of Inorganic Phosphate in Biologic Samples To find out the inorganic phosphate in a protein-containing liquid biological sample an aqueous reagent comprises an acid. This acid is capable of reacting with the molybdate salt to form molybdic acid for complexation with phosphate to form phosphomolybdate complexes, a molybdate salt, a nonionic surfactant reagent in an amount sufficient to
Laboratory Techniques

TRICINE-SDS-PAGE Protocol

Introduction and Principle Proteins in the mass range 1–100 kDa are commonly separated by Tricine–SDS-PAGE. For the resolution of proteins less than 30 kDa, it is the preferred electrophoretic system. As compared to other electrophoretic systems, the concentrations of acrylamide used in the gels are lower. Electroblotting is facilitated by these lower concentrations, which is particularly crucial for hydrophobic proteins.