Day: June 26, 2019

Laboratory Techniques

Affinity Chromatography Protocol

Introduction Affinity chromatography is a versatile separation protocol that uses the biological interactions for characterization and detailed analysis of sample components. It is based on highly

Laboratory Techniques

BRADFORD Protein Determination Protocol

Need Laboratory Equipment for your reasearch? Click here Introduction and Principle Developed by Marion M. Bradford in 1976, the Bradford assay is a quick protocol, and

Laboratory Techniques

Fast protein liquid chromatography (FPLC) Protocol

Introduction Fast protein liquid chromatography (FPLC) is a type of liquid chromatography that provides high resolution by small-diameter stationary phases for protein characterization and separation. The

Filtration Chromatography Protocol
Laboratory Techniques

Gel Filtration Chromatography Protocol

Need benchtop equipment for your lab? Click here Introduction Gel-filtration chromatography, also known as size exclusion chromatography, is a versatile technique that permits the separation of

Laboratory Techniques

Ion-exchange Chromatography Protocol

Introduction Ion Exchange Chromatography (IEC) is a powerful liquid chromatographic technique used for bioseparation. The separation is done by a reversible interaction between charged molecules of

Laboratory Techniques

High performance liquid chromatography (HPLC) Protocol

Introduction High-performance liquid chromatography (HPLC) is a powerful separation technique used for the analysis of ions, proteins, and organic molecules. HPLC is based on the mechanisms

Laboratory Techniques

KJELDAHL Method Protocol

Introduction and Principle The Kjeldahl method was developed by a brewer called Johann Kjeldahl in 1883. The protocol is built on the principle that strong acid

Protein Quantification Protocols
Laboratory Techniques

Protein Quantification Protocols

Introduction: Quantitative Method The estimation of proteins via the quantitative method is one of the basic requirements in biochemistry. Proteins, from various perspectives, are substantially more

SDS-Polyacrylamide Gel Electrophoresis at Neutral pH (NuPAGE)
Laboratory Techniques

SDS-Polyacrylamide Gel Electrophoresis at Neutral pH (NuPAGE)

Introduction and Principle The traditional Laemmli system denaturing conditions are stimulated by the Invitrogen NuPAGE SDS-PAGE gel system which is a revolutionary high-performance polyacrylamide gel electrophoresis

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