Pre-anesthesia fasting of 8 to 12 hours is required in larger animals to minimize the risk of vomiting during induction or recovery from anesthesia. In smaller animals this step is unnecessary. However, guinea pigs may retain food in their pharynx. If this phenomenon is observed in a significant number of subjects (guinea pigs) then fasting them for 3 to 4 hours before anesthesia may be sufficient. Pre-anesthesia tests should also be performed to assess the subject’s health status and age.
Laryngoscopy can be performed in lightly anesthetized subjects; however, it is recommended that this method only be used once proficiency in the technique has been achieved. Before intubating, the subject should be administered oxygen for about 2 minutes as a preemptive measure to slow down the onset of hypoxia that could arise from the inadvertently obstructed larynx. Although laryngoscopy can be performed as an emergency diagnostic tool before surgical correction, it is most effectively performed in stable subjects.
- Place the subject on its back.
- Hold the laryngoscope in your dominant hand.
- Extend the subject’s tongue forward and to the left.
- Slowly insert the blade into the right side of the subject’s mouth.
- Advance the blade inward and midline towards the base of the tongue.
- Place the tip of the blade under the epiglottis.
- Apply pressure caudally and upward with the handle placed at an angle of 45 degrees.
- Lift the handle until vocal cords are visualized.
- Perform intubation under direct visualization of vocal cords.
- Withdraw the blade while firmly holding the endotracheal tube in place.
Remove blade from the handle and tighten bulb before rinsing the blade under cool running tap water to remove all visible soil. Scrub the blade thoroughly using a soft bristle blade after soaking the blades in an enzymatic detergent. Wash once again under cool running tap water to remove residual detergent. Dry the blade using a clean lint-free cloth.
Skeletal and soft tissue factors of the subjects may affect the visibility of the line of sight of the larynx. Improper usage of the blade can result in trauma to soft tissues and damage frontal teeth. When selecting blade size, consider the weight of the subject. Always test the laryngoscope blades and handles after cleaning, disinfection, sterilization and before usage. Since the handles of the blades also serve as counterbalance an appropriate size of the handle should be selected to match the blade size used.
Ayre, P. (1937). Anæsthesia for hare‐lip and cleft palate operations on babies. British Journal of Surgery, 25(97), 131–132
Flecknel, P. (2009). Laboratory Animal Anaesthesia. Elsevier.
Hunter, S. C., Glen, J. B., & Butcher, C. J. (1984). A modified anaesthetic vapour extraction system. Laboratory Animals, 18(1), 42–44