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Melissa Martinez

Molecular GeneticsScience
June 14, 2021

Cloning into Bacteriophage M13 Vectors

Insertion of foreign DNA into M13 cloning vectors is a bit tricky and includes the following steps: Ligating double-stranded DNA segments with cohesive termini into compatible sites in M13 double-stranded RF DNA. Transforming ligation products into competent male E. coli cells (plated in top agar supplemented with X-gal and IPTG. …