Melissa Martinez, Author at Conduct Science

Laboratory Techniques Science

March 29, 2021

Large Scale Extraction of Bacteriophage λ DNA Using Proteinase K

Large-scale extraction of phage DNA involves digestion of viral coat proteins using a strong protease such as proteinase K followed by extraction with chloroform. This method is suitable for small-scale preparations as well. In the experiment, dialysis of bacteriophage suspension is followed by the extraction of phage particles with chloroform:…

Melissa Martinez

Laboratory Techniques Science

March 29, 2021

Electroporation-based CRISPR Cas9 delivery

CRISPR Cas9 is a potent and easy-to-use tool for gene editing, but the delivery of the CRISPR Cas9 system has been a challenge since it was developed. Several methods have been developed to effectively transport components of the CRISPR Cas9 system into the cells. The protocol below describes the electroporation-based…

Melissa Martinez

Laboratory Techniques Science

March 29, 2021

Purification of Bacteriophage λ Arms using Sucrose Density Gradients

Replacement vectors or substitution vectors are prepared by removing the central filler segment of phage DNA to accommodate the fragments of foreign DNA. This procedure is known as "preparation of λ arms." Restriction endonucleases are used to separate the two arms of phage DNA from the central stuffer fragment. Following…

Melissa Martinez

Laboratory Techniques Science

March 28, 2021

Preparation of Bacteriophage λ DNA Cleaved with two Restriction Enzymes to be used as a Cloning Vector

At each end of the central stuffer fragment, a series of restriction sites arranged in opposite directions are present in replacement vectors. For instance, in EMBL3A, the order of restriction site in the left polyclonal site is SalI-BamHI-EcoRI, and that of the right polyclonal site is EcoRI-BamHI-SalI. Digestion of this…

Melissa Martinez

Laboratory Techniques Science

March 26, 2021

Alkaline Phosphatase Treatment of Bacteriophage λ Vector DNA

Alkaline phosphatase treatment is used to suppress the background of non-recombinants while performing restriction digestion with insertion vectors containing a single cloning site or polyclonal site using a single restriction enzyme. In this method, ligated DNA is first subjected to phenol: chloroform extraction and standard ethanol precipitation, respectively. It is…

Melissa Martinez

Conduct Lab Management

September 28, 2020

Best Aquarium, Reef Tank, and Pond Sterilizers for Sale

Aquarium, reef tank, and pond sterilizers are popular instruments in both home and professional settings. Whether it’s a submersible sterilizer or an external device, most sterilizers employ UV radiation to eliminate harmful microorganisms, such as algae. Note that UV radiation is one of the most effective disinfection methods utilized to…

Melissa Martinez

Conduct Lab Basics Science

September 22, 2020

sgRNA construction and design

Clustered regularly interspaced short palindromic repeats (CRISPR) technology is a rapid, cheap, and efficient tool to edit genomes at a precise location. CRISPR technology has now been reduced to just two components: Endonuclease and g RNA. Endonuclease makes an incision at the target DNA molecule to be edited, and gRNA…

Melissa Martinez

Conduct Resources Science

September 20, 2020

Pre-market Approval for Medical Devices

US is the largest market for medical devices, worth approximately $156 billion (as of 2017), and is expected to reach $208 billion by 2023. Given these numbers, it’s no surprise that high-risk medical devices must undergo strict regulatory and approval processes. One of them is the pre-market approval (PMA) for…

Melissa Martinez

Conduct Science Science of Politics

September 20, 2020

Science and Foreign Affairs: A Global Consolidation for a Better World

From climate change policies to pandemic preparedness plans, foreign relations impact people on an individual, national, and global levels. The world of international affairs is, in fact, a mixture of politics, culture, economics, and innovations, with science and technology being two of the main factors in improving multinational relations. Whether…

Melissa Martinez

Conduct Science

August 13, 2020

Recombinant DNA technology (Inserting foreign DNA in the host organism)

Recombinant DNA molecules are developed in the laboratory by bringing together genetic materials from multiple organisms to produce new genetic combinations that are not previously found in biological organisms. These new combinations are made to benefit science, medicine, agriculture, and industry (Khan, S et al., 2016). Stanford researcher Paul Berg,…

Melissa Martinez

Cart

Melissa Martinez

Large Scale Extraction of Bacteriophage λ DNA Using Proteinase K

Electroporation-based CRISPR Cas9 delivery

Purification of Bacteriophage λ Arms using Sucrose Density Gradients

Preparation of Bacteriophage λ DNA Cleaved with two Restriction Enzymes to be used as a Cloning Vector

Alkaline Phosphatase Treatment of Bacteriophage λ Vector DNA

Best Aquarium, Reef Tank, and Pond Sterilizers for Sale

sgRNA construction and design

Pre-market Approval for Medical Devices

Science and Foreign Affairs: A Global Consolidation for a Better World

Recombinant DNA technology (Inserting foreign DNA in the host organism)

Our Company

For Scientists

OUR PRODUCTS