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Allosteric Enzymes: Characteristics, Models, and Examples

Introduction: Allosteric enzymes are a group of biocatalysts that possess common characteristics of an enzyme but do not exhibit a typical Michaelis-Menten kinetic behavior. Instead, their kinetics follow a sigmoid curve. The key to allosteric enzyme activity lies in the reversible binding to a specific molecule on a non-catalytic site of the enzyme. The binding initiates the formation of enzyme-substrate
Molecular Genetics

Bacteriophage λ DNA Purification from Liquid Cultures

In the past, bacteriophage λ DNA purification was largely achieved through procedures that involved Cesium chloride gradients. However, these methods had a low recovery rate and a high chance of the presence of contaminants. Modern bacteriophage λ DNA purification methods give high yield and provide DNA free from contamination (Pickard, 2009). We can also purify bacteriophage λ DNA from liquid

Molecular Genetics

Bacteriophage λ DNA Purification from Plate Lysates

Introduction Genomic DNA or cDNA clones are analyzed in a simple procedure that begins with the restriction digestion of phage DNA mini preparations. The digested DNA is then assessed by agarose gel electrophoresis. For the rapid analysis, one can purify bacteriophage DNA from plate lysates as well as liquid cultures. This method explains the purification of phage DNA from plate
Molecular Genetics

Cloning into Bacteriophage M13 Vectors

Introduction and Principle Insertion of foreign DNA into M13 cloning vectors is a bit tricky and includes the following steps: Ligating double-stranded DNA segments with cohesive termini into compatible sites in M13 double-stranded RF DNA. Transforming ligation products into competent male E. coli cells (plated in top agar supplemented with X-gal and IPTG.  Picking and propagating recombinant (white) and non-recombinant
Molecular Genetics

Generation of Single-Stranded DNA with Phagemid Vectors

Introduction The vectors derived from filamentous phages containing a plasmid's origin of replication are called phagemids (Qi et al., 2012). Phagemids comprise typical high-copy-number plasmids equipped with a major intergenic region (508bp in length) of a filamentous phage. This region does not encode any proteins; however, it comprises all the cis-acting sequences required to initiate and terminate viral DNA synthesis.
Molecular Genetics

Hybridization based Screening of Bacteriophage DNA on the filters

Introduction Filters carrying immobilized bacteriophage DNA are labeled with 32P-labelled probes to screen by hybridization in situ. In this procedure, the filters are first submerged in a prehybridization solution (containing blocking solution) to reduce non-specific probe absorption. The filters are then incubated with a denatured radioactive probe for hybridization. Wash the filters to remove the non-specifically attached probe. Get the
Paul S. Monks - Featured Image

Breathomics: Looking Into Breath Analysis for Medical Diagnostics

Name: Paul S. Monks Location: Leicester, UK H-index: 49 Twitter followers: 2,236 Hello! Who are you and what are you working on? I’m Paul Monks, and I’m currently the Chief Scientific Advisor to one of the UK government’s departments, the Department of Business, Energy, and Industrial Strategy. I’m responsible for the role that science plays in supporting development and delivery


Amino Acids: Building Blocks of Proteins

Introduction and History Amino acids are organic compounds consisting of carbon, hydrogen, and nitrogen. They are monomers or building blocks of proteins. Proteins are one of the major biomolecules required for the proper functioning of living organisms. Moreover, they are the first biomolecules that were first recognized for their biological roles in organisms than the other biomolecules including carbohydrates, nucleic

CSP: Apocalypse – Zombies

The Conduct Science Podcast: Apocalypse – Zombies 00:00 – Introduction 02:00 – News 10:30 – What are zombies? 16:05 – Favourite films 26:05 – Our zombie plan 32:50 – The science of zombies 1:02:00 – Ending and outro You can listen to The Conduct Science Podcast by using the player above, searching for “The Conduct Science Podcast” on any place

Arpit Kumar Pradhan - Featured Image

Neurodegeneration: Insights into Alzheimer’s and Bioinformatics

Name: Arpit Kumar Pradhan Number of lab members or colleagues (excluding PI): 9 Location: Munich, Germany Graduation Date: 01/05/2019 Grants: 4 H-index: 2 Hello! Who are you and what are you working on? I am Arpit and I am currently working as a clinical scientist at Klinikum Rechts der Isar, Technische Universität München. I work in the field of neurodegeneration.