Laboratory Techniques

Laboratory TechniquesProtocolsScience
April 13, 2020

Biomolecular NMR Spectroscopy

The information about the structure, interactions, and dynamics of biomolecules (protein, DNA, and RNA) is essential for understanding cellular processes. This knowledge is deconvolved by scientists and used in elucidating cellular mechanisms for finding cures for human diseases. The pharmaceutical industry, for example, uses this structural information to improve on…
The light digital microscope can be used to visualize structures as small as ~1 micron
Lab BasicsLaboratory TechniquesScience
March 9, 2020

Bright field microscopy

Microscopy is used to visualize objects that are too small to see with the naked eye. In biology, this technique enables us to examine things like bacteria and cells at a magnification of up to 10000 times their original size (BIO1000F practical manual, 2017). In light microscopy, visible light is…
Laboratory TechniquesScience
February 24, 2020

Spin-Spin coupling in NMR

The structure of a molecule can be predicted using NMR spectroscopy. However, the interpretation of the signals in an NMR spectrum relies on several factors. One of the factors affecting the location of the peaks in an NMR spectrum is Chemical shift. The location of the peaks is important in…
Laboratory TechniquesScience
February 17, 2020

Histochemical techniques to demonstrate Carbohydrates-Polysaccharides

Carbohydrates are the most abundant and one of the four essential macromolecules, required for the survival of living beings. Structurally, these are polyhydroxy aldehydes or ketones. Carbohydrates are divided into three classes depending upon the number of forming units (aldehyde or ketone), which are monosaccharides, oligosaccharides, and polysaccharides. These carbohydrates…
Laboratory Techniques
February 11, 2020

Histochemical demonstration of Acidic Compounds

As an Amazon Associate, ConductScience Inc. earns revenue from qualifying purchases Introduction The cell is composed of molecules of different chemical nature, whether they are acidic, basic, or neutral, all contribute to the functioning of the cell. Here, we will discuss the histochemical methods used to demonstrate the acidic components…