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Laboratory Techniques

Spectrophotometer
Laboratory TechniquesScience
February 24, 2020

Spin-Spin coupling in NMR

The structure of a molecule can be predicted using NMR spectroscopy. However, the interpretation of the signals in an NMR spectrum relies on several factors. One of the factors affecting the location of the peaks in an NMR spectrum is Chemical shift. The location of the peaks is important in…
Histochemistry
Laboratory TechniquesScience
February 17, 2020

Histochemical techniques to demonstrate Carbohydrates-Polysaccharides

Carbohydrates are the most abundant and one of the four essential macromolecules, required for the survival of living beings. Structurally, these are polyhydroxy aldehydes or ketones. Carbohydrates are divided into three classes depending upon the number of forming units (aldehyde or ketone), which are monosaccharides, oligosaccharides, and polysaccharides. These carbohydrates…
Histochemistry
Laboratory Techniques
February 11, 2020

Histochemical demonstration of Acidic Compounds

Introduction The cell is composed of molecules of different chemical nature, whether they are acidic, basic, or neutral, all contribute to the functioning of the cell. Here, we will discuss the histochemical methods used to demonstrate the acidic components of the cell which mainly include nucleic acids and some other…
radiobiology
Laboratory TechniquesProtocols
December 5, 2019

What is Autoradiography?

As an Amazon Associate, ConductScience Inc. earns revenue from qualifying purchases Autoradiography is an imaging technique that uses radioactive sources contained within the exposed sample. In vitro autoradiography methods involve the isolation of cellular components such as DNA, RNA, proteins or lipids, followed by labeling with suitable radioisotopes. In in…
Histochemistry
Lab BasicsLaboratory Techniques
December 2, 2019

Histochemical Methods For Protein Demonstration

As an Amazon Associate, ConductScience Inc. earns revenue from qualifying purchases Introduction Proteins are macromolecules that perform a vital role in every functional aspect of life processes, like transportation of molecules, signaling, movement of cells, regulation of gene expression, etc. Their purification, localization, and distribution have always been a critical…
Quinolinic acid rat HD model
Protocols
November 8, 2019

Quinolinic acid rat HD model

Huntington’s disease (HD) is an inherited autosomal dominant neuropathological disease caused by a mutation in the huntingtin gene (htt). Preclinical animal models are necessary for the study of HD and the development of therapeutic interventions for the disease. Ideally, an animal model should demonstrate all the behavioral and neuropathological characteristics…
3-Nitropreopionic acid Huntington’s disease model
Protocols
November 7, 2019

3-Nitropreopionic acid Huntington’s disease model

3-Nitropropionic acid (3-NP) is a selective striatal neurotoxin used in rodents to mimic pathological features of Huntington’s disease. 3-Nitropropionic acid interrupts with the mitochondrial electron transport chain. This mycotoxin is an inhibitor of succinate dehydrogenase, an enzyme responsible for the oxidation of succinate to fumarate. This process reduces ATP production…