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SDS-Polyacrylamide Gel Electrophoresis at Neutral pH (NuPAGE)
Laboratory Techniques

Capillary Electrophoresis

As an Amazon Associate, ConductScience Inc. earns revenue from qualifying purchases Capillary electrophoresis is one type of electrophoresis in which the separation of molecules is performed in a capillary tube that is immersed in an electrophoresis running solution. It is the only type of electrophoresis that can be modified to be fully-automated and to accommodate all forms of electrophoresis, this

Mastering the Microtome
Laboratory Techniques

The Cryostat-Microtome

A Glimpse into the History of a Cryostat A Cryostat is a versatile and high-quality machine that generates low temperatures for tissue sectioning. The word “Cryostat” originates from two separate Greek words “Kryos”, meaning cold, and “stat”, meaning stable. Sir James Dewar, Scottish Physicist, and Chemist are credited for the use of the very first cryostats in the 19th century.

Spectrophotometer
Laboratory Techniques

A Brief on The NMR Spectroscopy of Proteins

Spectroscopy is “the study of investigation and measurement of the absorption and emission of light or electromagnetic radiation by matter as a function of its wavelength and frequency”. In simple terms, it’s the study of the interaction of light with a matter or substance. Depending on the nature of the interaction between the energy and material, spectroscopy is divided into

Spectrophotometer
Laboratory Techniques

The Nuclear Overhauser Effect

Introduction The nuclear overhauser effect is defined as a change in the integrated intensity of one spin when the equilibrium population of another spin is perturbed by saturation or inversion. So, it is the transfer of nuclear spin polarization from one spin-active nucleus to another. However, the nuclei being irradiated should be closer to the other nuclei, in space, to

SDS-Polyacrylamide Gel Electrophoresis at Neutral pH (NuPAGE)
Laboratory Techniques

Polyacrylamide Gel Electrophoresis

As an Amazon Associate, ConductScience Inc. earns revenue from qualifying purchases Polyacrylamide gel electrophoresis is one of the first forms of gel electrophoresis. Polyacrylamide is a synthetic polymer that is considered the most all-around separation matrix to date due to its transparency, electrical neutrality, and adjustable pore size. The technique can be applied to the analysis of proteins and nucleic

2 Magnetic resonance imaging (MRI)
Laboratory Techniques

Magnetic Resonance Imaging (MRI)

Magnetic resonance imaging is an advanced, non-invasive diagnostic medical imaging technique that provides three-dimensional images of organs. The discovery of MRI involves a series of successful events that started in 1946 when Felix Bolch proposed the new magnetic properties of the atomic nucleus. He explained the behavior of atoms at tiny magnets, and based on these, NMR tools were developed.

SDS-Polyacrylamide Gel Electrophoresis at Neutral pH (NuPAGE)
Laboratory Techniques

Introduction to Preparative Electrophoresis

Electrophoresis techniques have been primarily developed for analytical purposes— to investigate the characteristics of the molecules in the sample of interest. The techniques can be modified to serve a preparative purpose, in which a large amount of a specific molecule can be obtained at the end of an electrophoresis run. Preparative electrophoresis uses the same principles as analytical electrophoresis but

Nucleic Acid Quantification Protocols
Laboratory Techniques

Drosophila: General Information and Methods for Experiments

Whoever thought that the small fly hovering over fruits will become such an essential asset for genetics research!! Fruit flies are one of the first extensively used model organisms in labs. Moreover, it is one of the first model organisms which was brought ‘from nature to labs’ to understand the genetics of the organisms! N. M. Stevens was the first

SDS-Polyacrylamide Gel Electrophoresis at Neutral pH (NuPAGE)
Laboratory Techniques

Cellulose Acetate Electrophoresis

Cellulose acetate electrophoresis is a type of zone electrophoresis that separates molecules in liquid form such as proteins on a membrane or stripe made of cellulose acetate. Developed by a German chemist, Joachim Kohn (1912-1987) in 1957, cellulose acetate electrophoresis was the second form of zone electrophoresis, after filter paper electrophoresis. It was also one of the earliest electrophoretic techniques

Nucleic Acid Quantification Protocols
Laboratory Techniques

Estimating the Chiasma Frequency in Diplotene-Diakinesis Stage

Chiasma is the point of crossing over or site where the exchange of genetic material takes place between two homologous, non-sister chromatids. The crossover occurs in the pachytene stage, however, it is observed in the diplotene stage of meiosis-I. The cross over between the two homologs also creates a new combination of parental genes, forming recombinants. The recombination of the