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Mastering the Microtome
Laboratory TechniquesProtocolsScience
April 20, 2021

The Cryostat-Microtome

A Glimpse into the History of a Cryostat A Cryostat is a versatile and high-quality machine that generates low temperatures for tissue sectioning. The word “Cryostat” originates from two separate Greek words “Kryos”, meaning cold, and “stat”, meaning stable. Sir James Dewar, Scottish Physicist, and Chemist are credited for the…
Spectrophotometer
Laboratory TechniquesProtocolsScience
April 5, 2021

The Nuclear Overhauser Effect

Introduction The nuclear overhauser effect is defined as a change in the integrated intensity of one spin when the equilibrium population of another spin is perturbed by saturation or inversion. So, it is the transfer of nuclear spin polarization from one spin-active nucleus to another. However, the nuclei being irradiated…
2 Magnetic resonance imaging (MRI)
Laboratory TechniquesProtocolsScience
March 15, 2021

Magnetic Resonance Imaging (MRI)

Magnetic resonance imaging is an advanced, non-invasive diagnostic medical imaging technique that provides three-dimensional images of organs. The discovery of MRI involves a series of successful events that started in 1946 when Felix Bolch proposed the new magnetic properties of the atomic nucleus. He explained the behavior of atoms at…
SDS-Polyacrylamide Gel Electrophoresis at Neutral pH (NuPAGE)
Laboratory TechniquesProtocolsScience
October 9, 2020

Cellulose Acetate Electrophoresis

Cellulose acetate electrophoresis is a type of zone electrophoresis that separates molecules in liquid form such as proteins on a membrane or stripe made of cellulose acetate. Developed by a German chemist, Joachim Kohn (1912-1987) in 1957, cellulose acetate electrophoresis was the second form of zone electrophoresis, after filter paper…