Category: Molecular Genetics

Molecular Genetics

Cloning into Bacteriophage M13 Vectors

Introduction and Principle Insertion of foreign DNA into M13 cloning vectors is a bit tricky and includes the following steps: Ligating double-stranded DNA segments with cohesive

Molecular Genetics

Generation of Single-Stranded DNA with Phagemid Vectors

Introduction The vectors derived from filamentous phages containing a plasmid’s origin of replication are called phagemids (Qi et al., 2012). Phagemids comprise typical high-copy-number plasmids equipped

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