Automated and Intelligent Stereotaxic Instrument

Specifications

Model	Product Description	Remarks
RWD-71000-S	Automated Stereotaxic Instrument, SGL.M*	Left manipulator, without laptop, adaptor, ear bars, holder and mask.
RWD-71000-D	Automated Stereotaxic Instrument, Dual.M*	Two manipulators and nanoliter injection pump included, without laptop, adaptor, ear bars, holder and mask.
RWD-71001-S	Automated Stereotaxic Instrument, Single.M, Automatic Injection*	Left manipulator and nanoliter injection pump included, without laptop, adaptor, ear bars, holder and mask.
RWD-71001-D	Automated Stereotaxic Instrument, Dual.M, Automatic Injection*	Two manipulators and nanoliter injection pump included, without laptop, adaptor, ear bars, holder and mask.

*Stereotaxic host (without accessories)

Accesories

Model	Product Description	Remark
RWD-68055	Mouse Adaptor
RWD-68030	Mouse/Neonatal Rat Adaptor	Recommended for Mice
RWD-68021	Rat Adaptor	Recommended for Rats
RWD-68057	Mouse Anesthesia Adaptor	No additional mask is required
RWD-68053	Rat Anesthesia Adaptor	No additional mask is required
RWD-68301	Rat 18° Ear Bars	Recommended for Rats
RWD-68306	Mouse 60° Ear Bars	Recommended for Mice

Additional Accesories

Model	Product Description	Remarks
RWD-TRA110	Tissue Removal Optional Kit (220V )	Optional when using tissue removal procedure, including needle connector, holder (68202), vacuum pump-GM-0.5Ⅱ -220V-50HZ, liquid storage tank, connecting tube, needle.
RWD-TRA-220	Tissue Removal Optional Kit (110V )	Optional when using tissue removal procedure, including needle connector, holder (68202), vacuum pump-GM-0.5Ⅱ -110V-50HZ, liquid storage tank, connecting tube, needle.
RWD-78001	Microdrill	For craniotomy. (Bits not included)
RWD-78040	Drill Bits HM1005 0.5mm, Round Tip, pkg of 5**
RWD-78042	Drill Bits HM1005 0.8mm, Round Tip, pkg of 5**
RWD-68605	Microdrill holder
RWD-71000-I	Injection Module for Automated Stereotaxic Instrument	Contains nanoliter injection pump + connecting wire + 3.5-inch glass capillary + 7-inch glass capillary + universal fixing accessories + 68207V-attachment + filling needle + sealing material + hexagonal wrench

Features

1µm Accuracy

Higher precision, suitable for injection of small nuclei

Software Control

Move at a constant speed to reduce damage.

Built-in Atlas

Know the probe position in real time.

Automated Craniotomy

Optional round or square cranial window

No Leveling Required

Precise positioning without leveling.

Single Point Injection

Control nanoliter syringe pump for automated injection.

Mutil-point Injection

Up to 10 points can be injected.

Tilted Injection

No need to manually calculate displacement after tilting.

Tissue Removal

Tissue removal suitable for calcium imaging experiments

Optional Dual Manipulators

Bilateral brain regions of the same animal can be located.

Needle Withdrawal Speed

The needle withdrawal speed can reach as low as 1μm/s.

One-click Movement

Move to target point or entry point with one click.

Information

High-precision stepping motor

The high-precision stepper motor stably outputs a minimum step angle of 0.04, which is a minimum displacement of 0.33μm, ensuring the accuracy requirement of 1μm. A high-precision projector is used to detect the accuracy of the motor to meet the needs of higher-precision experiments.

Anti-backlash function

Eliminate the gap error caused by the movement of the screw rod and achieve higher positioning accuracy.

Frame calibration

Quickly calibrate three-axis coordinate values, users can calibrate by themselves.

Constant Speed

The AP or ML axis has 5 adjustable speeds, the DV axis has 9 adjustable speeds, and the lowest speed in the DV direction can reach 1μm/s. Reduce jitter at a uniform speed to ensure precise positioning. Reduce mechanical damage. Prevent massive bleeding from affecting surgery.

Built-in Atlas

The software integrates the rat and mouse brain atlas, which can display the movement trajectory and position of the probe in real time and keep track of the experimental progress.

No Leveling Required

By determining four points to determine the skull tilt angle, accurate positioning can be achieved without leveling through angle calculation. (Bregma, Lambda, 2mm left and right of the sagittal suture) At the same time, the brain atlas can be scaled according to the actual skull size, making the positioning more accurate.

Automated Injection

The software directly controls the nanoliter syringe pump for single-point, multi-point and tilted injection. Tilted injection also does notrequire manual calculation of the three-axis displacement after tilting.  The injection precision is high, the injection volume is 0.6-5000nL, and the injection rate is 0.02-200nL/s. The needle withdrawal speed can be customized, with a minimum of 1μm/s. Slowly withdrawing the needle can prevent drugs or viruses from spilling into the needle tract. Multi-point injection procedure can achieve up to 10 injection sites. Needle retention time setting can realize automated needle retention and extraction. Real-time display of injection progress.

Tilted Trajectory: Manipulator Tilted Function

Traditional positioning methods require manual calculation of the three-axis displacement distance after tilting when performing experiments such as tilt injection or implantation, which is time-consuming and labor-intensive. The Automated Stereotaxic Instrument includes a tilted trajectory function, eliminating the need to manually calculate the displacement distance after tilting. It can be used for experiments such as tilted craniotomy, tilted injection and tilted implantation.

Optional Dual Manipulators

It can realize experiments such as injection and implantation of bilateral brain areas of the same animal, which is more efficient.

Automated Craniotomy: Skull Windows Function

You can choose a round or square window, which is suitable for brain imaging scenarios, such as miniscope calcium imaging experiments, two-photon imaging or other brain imaging experiments before craniotomy.

Tissue Removal

Equipped with a tissue removal kit, it is used to remove tissue before implanting the GRIN Lens. It is suitable for miniscope calcium imaging experiments. Compared with manual tissue aspiration, fully automated aspiration ensures flat wound end surfaces, less additional neuronal damage, and better imaging as compared to manual tissue removal.

Features

1. Choose a high-precision stepper motor drive, resolution 1μm, running accuracy up to 10μm
2. The output torque of the motor is large, which can drive other accessories attached to the locator
3. Save time, set the target position to move independently
4. Automatic record, display position parameters, reduce human error
5. The computer interface can display the trajectory and position of the probe in real-time
6. Can accurately control the depth of entry
7. Support four types of needle tubes: 30G, 29G, 28G, 27G
8. The depth, order, and repetition times of tissue extraction can be set through software
9. Bregma point can be set with one click;