$130.00 – $1,040.00
Basic Fuchsin is a fluorescent dye containing a mixture of rosaniline, pararosaniline, magenta II, and new fuchsine. The basic fuchsin is a cationic triphenylmethane dye used for the detection of acid-fast bacilli and is commonly used in the Ziehl Neelsen staining technique. It stains mucopolysaccharides and glycoproteins. It can also be used to track proteins in acidic pH systems. The basic fuchsin stain acts as a nuclear stain, elastic tissue stain, and mucin stain.
The basic fuchsin dye is dominantly used in staining procedures. It is used for Gomori’s aldehyde-fuchsin method, periodic acid Schiff method, and in Endo medium to distinguish E.coli from K.aerogenes bacteria. The basic fuchsin is also employed as a counter-stain in Gram’s staining.
A mixture of pararosaniline, rosaniline, new fuchsine, and magenta II makes basic fuchsin dye. Its chemical and physical properties are mentioned below:
The Basic fuchsin stain is used in a variety of staining methods including the Ziehl Neelsen technique, Taylor’s protocol, aldehyde fuchsin method, Mowry’s aldehyde fuchsin modification method, PAS method, and the Feulgen staining procedure. All these staining techniques have basic fuchsin in one of the solutions. The protocols followed for these staining techniques are discussed below:
This procedure is used to stain acid-fast bacilli. The mycolic acid in the cell membranes of the acid-fast bacilli retains the carbon fuchsin dye in this staining technique. The carbol fuchsin dye makes the bacteria appear red under the microscope. The staining protocol is described below:
The red-stained acid-fast bacilli with a blue background were analyzed under the microscope.
This staining technique is used for Gram-negative and Gram-positive bacteria. The staining procedure for Taylor’s method is described as follows:
The Gram-positive bacteria appear blue (filaments), while Gram-negative bacteria look red (nucleus), and additional tissue elements appear yellow under the microscope.
This staining technique is used to stain elastic fibers and pancreatic islet beta cells. The method follows the protocol mentioned below:
The pancreatic beta cells appear purple to violet with a green background under the microscope. The elastic tissues also look purple to violet after staining with the aldehyde-fuchsin solution.
The PAS stain uses periodic acid which oxidizes the carbon-carbon bond forming aldehydes. These aldehydes then react to fuchsin-sulfurous acid making a magenta color. The basement membranes, glycogen, undifferentiated squamous cell carcinoma, and mucosubstances secreted from the epithelia of different organs are distinguished and demonstrated using the PAS method. Kidney and liver biopsies also use the PAS staining technique. The procedure is discussed below:
The fungus and glycogen appear magenta with blue stained nuclei.
The Feulgen method is used to specify chromosomes and DNA in nuclei. The method employs Schiff’s reagent for staining. The protocol followed in this technique is described below:
The basic fuchsin staining technique can be used for histological and morphological analysis of a variety of human tissues, surgical specimens, and plant tissues. Also, the staining method is the most promising staining technique used for the examination of acid-fast bacteria, gram-positive and gram-negative bacteria, hepatitis B surface antigen (HBsAg), and sections of the pancreas, kidney, liver, and small intestine. Following are some of the research applications of the basic fuchsin staining method.
Camp and Liming utilized the basic fuchsin dye to trace the vascular bundles of herbaceous plants and the water-conducting system of woody plants. In the experiment, a stock solution of 2.5% basic fuchsin in 95% alcohol was used. The tissue specimens were observed under the microscope for histological and morphological examination. The basic fuchsin dye enabled the researchers to analyze plant anatomy by increasing the contrast in the sample slides.
In the study, the basic fuchsin dye was used to visualize lignin in the basal internodes of three Brachypodium distachyon ecotypes displaying different flowering times and analyze them using the fluorescent imaging technology. With the help of the basic fuchsin staining, the subcellular patterns of lignin in vascular and interfascicular fiber cell walls were visualized. The basic fuchsin dye did not quantify the lignin content in the subcellular compartments but provided the researchers with a new comparative tool for lignin imaging. This research also indicated that the Basic Fuchsin fluorescence is directly proportional to the amount of lignin between samples, stains hydroxycinnamates, and maybe inconsiderate to lignin composition.
Scheres and Merkx (1976) used the basic fuchsin stain to perform human metaphase chromosome banding. The cationic triphenylmethane dye specified the G-banding pattern’s main characteristics: the secondary constriction regions of chromosomes No. 1 and 16, the heterochromatic area of chromosome No. 9, and a number of chromosomal regions with bright Q fluorescence, the polymorphic regions of chromosomes No. 3, 4, and Y clearly. The research indicated that the basic fuchsin staining technique is suitable for telomeres, family, and linkages study.
The basic fuchsin dye is an excellent stain to study the nuclei of fungi. In this study, aqueous basic fuchsin was tested on a Feulgen-weak fungus, Blastomyces dermatitidis, and other fungi. Formaldehyde acted as a mordant to stain the samples permanently. The results suggested that the basic fuchsin is the most promising stain for the morphological and chemical analysis of fungi nuclei.
The basic fuchsin has been widely used for staining purposes in biomedical research. Following are the advantages and weaknesses of the basic fuchsin staining.
Following precautionary measures should be taken while handling the basic fuchsin dye.
4 x 1 gallon/case, 250 ml
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