Chemiluminescence Imaging System

Introduction

SB-SCG-W3000 is an imaging device that utilizes chemiluminescence technology and is equipped with a high-sensitivity cooled camera with 9 million pixels. It enables fast, accurate, and high-throughput detection and imaging of samples, and is widely used in fields such as life sciences, medicine, and environmental protection.

Principle

A Chemiluminescence Imaging System operates on the principle of detecting light emitted during a chemical reaction. Chemiluminescence occurs when a chemical reaction produces an excited state intermediate, which then releases energy in the form of light as it returns to a lower energy state. This light emission is captured by the imaging system, typically consisting of a highly sensitive camera (often a cooled CCD or CMOS sensor) and optical components that guide and focus the light onto the detector.

Specifications

Camera

Product	Remarks
PixelResolution	9 million pixels
Resolution	3000×3000
Pixel Size	3.76μm×3.76μm
Target Size	1 “(11.28mm×11.28mm)
Full Well Capacity	16.5ke-(HCG),50.5ke-(LCG)
Sensitivity	877mv@1/30s
Readout Noise	1.24e-(HCG), 3.22e-(LCG)
Dark Current	0.0003e-/s/pixel@-15℃
Signal-to-Noise Ratio	42.2dB(HCG), 47dB(LCG)
Exposure Time	0.1ms~1h
Binning Mode	1×1, 2×2, 3×3
Grayscale	16-bit (65536 levels)
Cooling	Relative to Ambient Temperature-40℃
Camera Type	Black and White Camera

Lens

Product	Remarks
Aperture	F0.95-F16
Focal Length	17mm
Type	Fixed Focus Lens

Light Source

Product	Remarks
Bright Field Light Source	Downward-facing LED white light source, symmetrically distributed on both sides

Dark Box

Product	Quantity
Light Isolation	Fully light-sealed, isolates environmental light.
Door Control	Door control sensor can control the on/off of the bright field light source
Field of View	Effective field of view is 136mm*136mm (expandable to 200mm×200mm if needed).

Software  Functions

Product	Quantity
Software Functions	Real-time Imaging: Real-time presentation of changes in sample signals during exposure process, capturing every detail of the capture
Overexposed areas can be displayed.	After exposure, each frame image within the exposure time can be generated. Users can select any frame image for the final output by precise retrospective adjustment.
Time Imaging	For samples with insufficient exposure, users can choose to continue exposure after the exposure ends, allowing the sample to gain additional exposure effect based on the already exposed time.
Auto Exposure	Intelligent exposure technology quickly determines the optimal exposure time. With time imaging and time accumulation functions, users can obtain the best image result with just one operation.

Apparatus and Equipment

• Real-time Imaging: Present the changes in sample signals during the exposure process in real-time, capturing every detail of the shot. Overexposed areas will be indicated for samples that are overexposed.
• Time Imaging: After the exposure is completed, every frame image within the exposure time can be generated. Through precise retrospective adjustments, users can select any frame image within that exposure time as the final output.
• Time Accumulation: For samples with insufficient exposure, users can choose to continue the exposure after the exposure ends, allowing the sample to obtain additional exposure effects based on the exposure time already accumulated.
• Automatic Exposure: Intelligent exposure technology can quickly determine the optimal exposure time. With the functions of time imaging and time accumulation, users can obtain the best image results with just one operation.

Protocol

1. System Start-Up and Sample Loading Begin by connecting the power cord and activating the power switch located at the back of the instrument. This will initiate the startup of the industrial computer, which will automatically load the chemiluminescence imaging software. Once the software interface appears, open the instrument door and remove the sample tray. Carefully place the prepared sample on the tray, ensuring it lies flat, and return the tray into the instrument’s dark box before closing the door.
2. Software Operation and Image Capture Upon successful software launch, confirm that all system components are functioning correctly by checking the status bar at the bottom left of the screen. If the system passes the self-check, proceed to the preview and capture page by selecting the central icon. On this page, configure the storage location for your experiment results and choose between manual or automatic exposure modes. Adjust exposure settings as needed and start the preview. Once satisfied with the setup, click the capture button to begin the imaging process. After capture, review the images, make any necessary adjustments, and save the final results.

Experimental Options and versatility

Applications

Chemiluminescence Imaging Systems are widely used in various scientific applications due to their high sensitivity and ability to localize and quantify light emission at very low levels. Here are the primary applications based on the scientific articles provided:

1. Biological Imaging:
   • In Vitro Assays: Chemiluminescence imaging is used for quantitative assays in microtiter plates, microarrays, and miniaturized analytical devices. It enables the simultaneous analysis of multiple analytes in a sample, making it suitable for high-throughput screening (HTS) in pharmaceutical and clinical research​ Roda et al., 2005 
   • In Vivo Imaging: These systems are essential for imaging biological processes in live animals, allowing researchers to track the localization and dynamics of specific molecules or cells over time. This is particularly useful in studying disease progression, drug delivery, and gene expression​ Roda et al., 2005 
2. Immunohistochemistry and In Situ Hybridization:
   • Chemiluminescence imaging is employed in immunohistochemistry and in situ hybridization to localize and quantify specific proteins, antigens, or gene sequences in tissue samples. The high sensitivity of these systems makes them ideal for detecting low-abundance targets​
3. Environmental and Pharmaceutical Analysis:
   • The technology is used in environmental monitoring, such as detecting pollutants like mercury in water samples using luminescent bacterial biosensors. In pharmaceutical applications, chemiluminescence imaging facilitates drug discovery by enabling the rapid screening of potential drug candidates​. Roda et al., 2005 
4. Whole-Organ and Whole-Body Imaging:
   • In preclinical research, chemiluminescence imaging is applied to visualize whole organs or entire organisms. This application is crucial for evaluating the distribution and effects of drugs, as well as for understanding complex biological systems in real time​ Roda et al., 2005 

These applications highlight the versatility and importance of Chemiluminescence Imaging Systems in advancing scientific research across multiple disciplines.

References

Roda, Aldo & Guardigli, Massimo & Pasini, Paolo & Mirasoli, Mara & Michelini, Elisa & Musiani, Monica. (2005). Bio and chemiluminescence imaging in analytical chemistry. Analytica Chimica Acta - ANAL CHIM ACTA. 541. 25-35. 10.1016/j.aca.2004.11.083. Wang, Zhijia & Huang, Jiaguo & Huang, Jingsheng & Yu, Bingran & Pu, Kanyi & Xu, Fu‐Jian. (2021). Chemiluminescence: From mechanism to applications in biological imaging and therapy. Aggregate. 2. 10.1002/agt2.140.