$85.00 – $413.00
Packaging: 4 bottles/case
For Gram Stains
Crystal violet is an organic chloride salt that belongs to the triphenylmethane type alkaline dyes. This monochloride salt whose scientific name is hexamethyl chloride rose aniline has a wide bio and industrial applications including developing fingerprints, dying ink, as an antifungal, antibacterial & anthelmintic agent, and as a histological stain. Other names used to describe crystal violet include Basic Violet 3, Gentian Violet, Hexamethyipararosaline chloride, and Methyl Violet 10B.
Its powder form appears as a dark green compound with metallic luster but appears purple when dissolved. When in an aqueous or alcohol solution, crystal violet has a (purple) blue-violet color, with the color largely depending on the acidity of the solution. The different colors of the dye are because of the different charged states of the dye molecule.
Charles Lauth was the first scientist to synthesize methyl violet in 1866. Crystal violet is one of the components of methyl violet. Crystal violet was first independently synthesized by Alfred Kern while working in Basel. For the easier synthesis of this product, he entered into a collaboration with a German chemist Heinrich Caro. Kern also discovered that diethylaniline could be used to synthesize violet dye, which is commonly known as Basic violet 4.
Jakob Stilling, a German ophthalmologist, discovered the antiseptic properties of crystal violet in 1890. He collaborated with E. Merck & Co. to market the dye, which was probably a mixture of aniline dyes, as an antiseptic. Later in 1902, Conradi and Drigalski discovered that the effect of the dye was strong on most gram-positive bacteria but had little effect on gram-negative bacteria such as Bacillus Typhi (Salmonella typhi) and Bacillus Coli (Escherichia coli). John Churchman published a more detailed study of the dye on the different strains of bacteria in 1912.
Some of the chemical properties of crystal violet include:
Crystal violet is prepared by using N, N-dimethylaniline. This product is condensed, chlorinated, and added taken through other reactions.
It can also be synthesized via the reaction of N, N-dimethylaniline, and Michler ketone in the presence of phosphorus oxychloride. It is then taken through an azeotropic reaction with hydrochloric acid. After this, the compound is then recrystallized in hot water to generate the compound.
No sterilization procedures are required in this process. The crystal violet powder is dissolved into the distilled water; methanol is then added to this solution.
The crystal violet solution made should them be stored in the dark at room temperature. It should be used within 2 months.
Crystal violet can be used to examine the effect of chemotherapeutics and other agents on growth and survival cells in a culture. Since during cell death, adherent cells normally detach from culture plates, this can be used to show differences in proliferation and quantify cell death on stimulation with death-inducing agents. Crystal violet binds to DNA and proteins in cells and as such can be used to detect the maintained adherence of cells. In this procedure, the dye works as an intercalating dye that enables the quantification of DNA which is always held proportional to the number of cells in the culture. The staining protocol takes about 6 minutes to perform. Crystal violet aids in the visualization of cells by staining the cell nuclei a deep purple color.
The staining procedure:
As an antibacterial and antifungal agent
Crystal violet can be used to kill or slow the growth of bacteria. It can also be used to destroy fungi by inhibiting their reproduction or ability to grow.
As an antiseptic drug
Crystal violet can be used as a disinfectant to inhibit the activity of microbes or to destroy them.
As an anthelminthic drug
Crystal violet can also be used to kill parasitic worms.
As a histological dye
Crystal violet is also used in the microscopic examination of cells to highlight and give contrast to specific features of the cell, e.g. nuclei and cytoplasm.
Crystal violet is useful in assessing or detecting bacterial contamination in tissue samples.
It is also essential in assessing the cytotoxic/cytostatic effects on tumor cell lines as well as testing the different species of archaea.
Crystal violet can lead to permanent pigmentation of the skin if it comes into contact with granulation tissue. On account of this, the dye should not be applied to ulcerative lesions of the face.
Crystal violet is not a strong sensitizer when used as an antiseptic agent.
Crystal violet may produce allergic contact dermatitis or necrosis in the intertriginous areas.
Its use for bacterial and pathogenic fungi treatment in animals such as Staphylococcus & Candida species has declined on account of reports on animal carcinogenicity.
Crystal violet is toxic and should be handled with a lot of care.
When using crystal violet to stain cells, the examiner ought to pipette on the sides of the petri dish so as to reduce the chances of the cells detaching.
Several toxicology tests carried out in mice have shown dose-related carcinogenic properties on different organs. The reduced leuco moiety form induces hepatic, renal, and lung tumors in mice. The FDA has classified crystal violet as unsafe for use in animal feed. The Federal Food, Drug, and Cosmetic Act prohibits its use in animal feed, and such feed is regarded as adulterated. Crystal violet is absorbed from water into the fish tissue and is then reduced into the leucocrystal violet (leuco) moiety.
A common side effect of crystal violet is the staining of skin and cloth; this can, however, be easily washed off by bleach and water.
4 x 1 gallon/case, 8oz