Weight Drop TBI models are used to replicating focal cerebral contusions as well as traumatic, diffuse brain injury by axonal damage. Attach a metal loop to the top end of the desired weight allowing the fishing line to be fastened to the weight. The plastic guide tube is held in place with a clamp stand and positioned above the scored tin foil. The fishing line is then attached through the metal loop to the weight ensuring that the bottom of the weight hangs freely over the scored tin foil. The fishing line is then connected to the clamp stand, and the weight is drawn up through the plastic guide tube with the fishing line and secured in its position with an Allen key pin.
Ferret Restrainers are constructed using optical quality clear acrylic, formed black plastic nose cone with an acetal locking slide. The restraining cylinder can be rotated as needed to provide complete access to the animal. The restrainers are mounted on a white acrylic base plate with suction cup feet to guarantee reliable stability during testing procedures.
Open Field test is a popular protocol used to assess exploratory behavior and anxiety. Thigmotaxis in the open field is used to evaluate anxiolytic, anxiogenic and even non-pharmacological treatments. Ambulation is the most common behavior studied with this maze, but others such as latency or rearing can also be measured. In addition, objects can be added for a modification similar to the novel object recognition field.
Walls can be both opaque or clear. We recommend that clear walls are used for measurements of anxiety, while opaque walls are used if assessments include novel object recognition.