Eosin Azure 65 is a cytological counterstain used for non-gynecological cytodiagnosis in Papanicolaou staining technique. The cells to be examined in cytodiagnosis could be of animal, human or plant origin. In human cytodiagnosis, the experiment can be further categorized into gynecological and non-gynecological. Non-gynecological samples are collected from urine, sputum, bronchial secretion, or ascites that contains squamous epithelial cells or other such cells. Through cytodiagnosis, the specimen collected can be examined for the presence of premalignant and malignant cells. After this examination, the cells are classified as normal, suspected, inflammatory, uncertain or malignant cells.
Dr. GN Papanicolaou first developed the Papanicolaou stain in 1947, and since then it has been successfully used for cervical cancer diagnosis. It has also reduced the incidence of cervical cancer by 70% particularly in developed countries allowing well-planned screening procedures. However, the stain has been modified from the conventional regressive method to progressive rapid Pap staining to reduce the duration of the procedure. Later the other modifications were Enviro- Pap stain to make the dye environment-friendly; REAP stain which was rapid and economical, and Cytocolor in which isopropyl alcohol was replaced by Propanol.
Papanicolaou defined the main objectives of staining the cytological smears as follows:
The differentiation of cells is important for the identification of the different cell types found in cytological smears.
Nuclear detail definition
Since there are many nuclear abnormalities in cancerous cells, appropriate staining of the nucleus is important for an accurate diagnosis.
Transparency of the cytoplasm is of particular importance because of the frequent overlapping of cells and their varying thickness.
Strengths and Limitations
EA-65 Stain is a stable and reliable dye for the identification and examination of non-gynecological cells for neoplasia and cancer. The dye reacts exclusively with the intended cells aiding in their identification and accurate diagnosis after examining the smear preparation. The diagnostic tests are not always accurate and at times could lead to false results.
- EA-65 Stain is a highly flammable liquid keeps it away from spark and hot surfaces.
- May cause eye and skin irritation, seek medical treatment in case of contact.
- Do not breathe vapors, spray or mist as it may cause target organ toxicity if inhaled.
- Do not smoke, eat or drink when using this product.
- Papanicolaou (Pap) staining is a cytological technique used to differentiate cells in smear preparations.
- The technique is reliable for cell differentiation from various bodily secretions such as urine, abdominal fluid, gynecological smears, tumor samples, and seminal fluid.
- EA-65 Stain is a significant component of the Papanicolaou stain, a procedure that is useful in cancer screening and diagnosis.