Elisa kit for Angiotensin 2

Our Elisa Kit products are perfect for the analysis of biological fluids, including Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids.

Elisa Kit for Angiotensin 2 has high sensitivity and excellent specificity for the detection of Angiotensin II (AngII). ConductScience offers Elisa Kits for the analysis of biological fluids.

$957.00
Specificity

This assay has high sensitivity and excellent specificity for the detection of Angiotensin II (AngII).

No significant cross-reactivity or interference between Angiotensin II (AngII) and analogs was observed.

Recovery

Matrices listed below were spiked with a certain level of recombinant Angiotensin II (AngII). The recovery rates were calculated by comparing the measured value to the expected amount of Angiotensin II (AngII) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)86-10290
EDTA plasma(n=5)83-9791
heparin plasma(n=5)79-9678
Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle, and high-level Angiotensin II (AngII) were tested 20 times on one plate, respectively. 

Inter-assay Precision (Precision between assays): 3 samples with low, middle, and high-level Angiotensin II (AngII) were tested on 3 different plates, with 8 replicates in each plate. 

CV(%) = SD/meanX100 

Intra-Assay: CV<10% 

Inter-assay: CV<12% 

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Angiotensin II (AngII) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)82-104%97-105%88-104%94-101%
EDTA plasma(n=5)96-105%90-97%91-99%96-103%
heparin plasma(n=5)92-102%79-92%97-104%93-101%
Stability

The loss rate of activity determines the stability of the kit. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. 

To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Packaging & Shipping

Reagents and materials provided
Sample1:21:41:81:16
serum(n=5)82-104%97-105%88-104%94-101%
EDTA plasma(n=5)96-105%90-97%91-99%96-103%
heparin plasma(n=5)92-102%79-92%97-104%93-101%
Assay procedure summary
  1. Prepare all reagents, samples, and standards;
  2. Add 100µL standard or sample to each well. Incubate 1 hour at 37°C;
  3. Aspirate and add 100µL prepared Detection Reagent A. Incubate for 1 hour at 37°C;
  4. Aspirate and wash 3 times;
  5. Add 100µL prepared Detection Reagent B. Incubate for 30 minutes at 37°C;
  6. Aspirate and wash 5 times;
  7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
  8. Add 50µL Stop Solution. Read at 450nm immediately. 
References

Magazine

Citations

Nature Medicine

Angiotensin-Converting Enzyme 2 Overexpression Remarkably Ameliorated Glomerular Injury in a Rat Model of Diabetic Nephropathy: A Comparison with ACE Inhibition MolMed: 10_11_liu

The European Journal of Clinical Investigation

Mesangial medium with IgA1 from IgA nephropathy inhibits nephrin expression in mouse podocytes Wiley: source

Immunopharmacology and Inflammation

Evidence for the complementary and synergistic effects of the three-alkaloid combination regimen containing berberine, hypaconitine, and skimmianine on the ulcerative colitis rats induced by trinitrobenzene-sulfonic acid ScienceDirect: S0188440910001049

Nephrology

Effect of aggregated immunoglobulin A1 from immunoglobulin A nephropathy patients on nephrin expression in podocytes Wiley: source

PLoS One

Angiotensin II and Angiotensin-(1-7) in Paraventricular Nucleus Modulate Cardiac Sympathetic Afferent Reflex in Renovascular Hypertensive Rats PubMed: PMC3527547

BioMed Research International

Heterogeneous Downregulation of Angiotensin II AT1-A and AT1-B Receptors in Arterioles in STZ-Induced Diabetic Rat Kidneys Hindawi: 947506

34

Biglcan is beneficial in angiotensin II induced heart failure by preventing cardiac inflammation and remodeling improving LV function and mortality by preventing transdifferentiation of myofibroblasts Oxfordjournals: Source

Pflügers Archiv-European Journal of Physiology

SOD1 gene transfer into paraventricular nucleus attenuates hypertension and sympathetic activity in spontaneously hypertensive rats Pubmed: 23114721

Eur J Pharmacol.

Angiotensin II and vasopressin are involved in the defense system against anaphylactic hypotension in anesthetized rats Pubmed:24650734

J Mol Cell Cardiol.

Increased LDL electronegativity in chronic kidney disease disrupts calcium homeostasis resulting in cardiac dysfunction Pubmed:25871829

Mol Med Rep.

High glucose induces activation of the local renin?angiotensin system in glomerular endothelial cells Pubmed:24337709

Mol Med Rep.

Angiotensin II promotes the progression of human gastric cancer Pubmed:24424956

PLOS ONE

Intermedin in the Paraventricular Nucleus Attenuates Cardiac Sympathetic Afferent Reflex in Chronic Heart Failure Rats Pubmed:24709972

Journal of Vascular Surgery

Cathepsin G deficiency reduces periaortic calcium chloride injury-induced abdominal aortic aneurysms in mice Pubmed:25037606

Biochim Biophys Acta.

Cathepsin G activity lowers plasma LDL and reduces atherosclerosis Pubmed:25092171

Acta Physiologica

Superoxide anions in the paraventricular nucleus mediate cardiac sympathetic afferent reflex in insulin resistance rats Pubmed:25307720

Neuroscience Letters

Administration of exogenous 1,25(OH)2D3 normalizes overactivation of the central renin-angiotensin system in 1α(OH)ase knockout mice Pubmed:25576706

34

A background Ca2+ entry pathway mediated by TRPC1/TRPC4 is critical for the development of pathological cardiac remodeling PubMed: 26069213

Life Sci

Cardioprotective effects of baicalein on heart failure via modulation of Ca2 PubMed: 26706290

Physiological Research

Renal Sympathetic Denervation Improves Cardiac Dysfunction PubMed: 25804096

Nutrition

Effects of Rubus occidentalis extract on blood pressure in subjects with prehypertension: a randomized, double-blinded, placebo-controlled clinical trial PubMed: 26740254

J Am Soc Hypertens

Angiotensin-(1–7) enhances the effects of angiotensin II on the cardiac sympathetic afferent reflex and sympathetic activity in the rostral ventrolateral medulla in renovascular hypertensive rats PubMed: 26428223

PLoS One.

Up ReCavia (Guinea pig )location of cystathionine γ lyase and Hydrogen Sulphide in the Myocardium Inhibits the Progression of Isoproterenol–Caffeine Induced Left Ventricular Hypertrophy in Wistar Kyoto Rats Pubmed:26963622

Nutrition

Effects of Rubus occidentalis extract on blood pressure in patients with prehypertension: Randomized, double-blinded, placebo-controlled clinical trial Pubmed:26740254

Journal of Atherosclerosis and Thrombosis

Involvement of Angiotensin II Type 1 and 2 Receptors in Gelatinase ReCavia (Guinea pig )lation in Human Carotid Atheroma in vitro Pubmed:26947595

Mol Neurobiol

A Therapeutic Insight of Niacin and Coenzyme Q10 Against Diabetic Encephalopathy in Rats Pubmed:26867655

Life Sci

Cardioprotective effects of baicalein on heart failure via modulation of Ca(2+) handling proteins in vivo and in vitro Pubmed:26706290

PLoS One.

Paradoxical Sleep Deprivation Causes Cardiac Dysfunction and the Impairment Is Attenuated by Resistance Training. pubmed:27880816

Oxidative Medicine and Cellular Longevity

Epigallocatechin-3-gallate Attenuates Renal Damage by Suppressing Oxidative Stress in Diabetic db/db Mice pubmed:27698952

Journal of the Renin-Angiotensin-Aldosterone System

Angiotensin type 2 receptor null mice express reduced levels of renal angiotensin-converting enzyme-2/angiotensin (1-7)/Mas receptor and exhibit greater high-fat diet-induced kidney injury 1470320316661871. abstract

Microvascular Research

Pleiotropic effects of survivin in vascular endothelial cells. pubmed:27373518

PLOS ONE

Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated withActivation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension. pubmed:27391973

journal of pharmacological sciences

β-Aminoisobutyric acid ameliorates the renal fibrosis in mouse obstructed kidneys via inhibitionof renal fibroblast activation and fibrosis. pubmed:28433566

Experimental Animals

Ginsenoside Rb1 improves cardiac function and remodeling in heart failure. pubmed:28367863

Oncotarget

Effects of renal denervation on monocrotaline induced pulmonary remodeling pubmed:28187460

The American Journal of Pathology

The Renin-Angiotensin System Regulates Neurodegeneration in a Mouse Model of Optic Neuritis pubmed:28919108

PLoS One

Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathy pubmed:29240788

Frontiers in Physiology

Hydrogen Sulfide Attenuates Atherosclerosis in a Partially Ligated Carotid Artery Mouse model via Regulating Angiotensin Converting Enzyme 2 Expression pubmed:29066981

Neurotherapeutics

Brain Renin–Angiotensin System Blockade Attenuates Methamphetamine-Induced Hyperlocomotion and Neurotoxicity Pubmed:29464572

International Journal of Cardiology

ALDH2 protects against alcoholic cardiomyopathy through a mechanism involving the p38 MAPK/CREB pathway and local renin-angiotensin system inhibition in … Pubmed:29506687

Neuroscience

Angiotensin II Type 1a Receptors in the Subfornical Organ Modulate Neuroinflammation in the Hypothalamic Paraventricular Nucleus in Heart Failure Rats Pubmed:29684507

American Journal of Physiology-Renal Physiology

Role of (Pro) Renin Receptor in Albumin Overload-Induced Nephropathy in Rats Pubmed:29846109

Journal of the American Society of Hypertension

Mesenchymal stem cell–derived microvesicles alleviate pulmonary arterial hypertension by regulating renin-angiotensin system Pubmed:29752040

Evidence-based Complementary and Alternative Medicine 

Acute Biochemical, Cardiovascular, and Autonomic Response to Hyperbaric (4 atm) Exposure in Healthy Subjects Pubmed:29977313

Brazilian Journal of Medical and Biological Research

Renovascular hypertension increases serum TNF and CX3CL1 in experimental Trypanosoma cruzi infection Pubmed:29590257

American Journal of Physiology-Heart and Circulatory Physiology

Sex Differences in the Central and Peripheral Manifestations of Ischemia-induced Heart Failure in Rats Pubmed: 30289294

Toxicology Mechanisms and Methods

Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar rats Pubmed: 30426860

Journal of Clinical and Aesthetic Dermatology

Detection of Angiotensin II and AT1 Receptor Concentrations in Keloid and Hypertrophic Scar.

International Journal of Ophthalmology

Serum microRNA-221 as a biomarker for diabetic retinopathy in patients associated with type 2 diabetes Pubmed: 30588418

JOURNAL OF STRENGTH AND CONDITIONING RESEARCH

ACE Gene Plays a Key Role in Reducing Blood Pressure in The Hyperintensive Elderly After Resistance Training Pubmed: 30908459

The American Journal of the Medical Sciences

T lymphocyte-based renin angiotensin system in obesity

Journal of Alzheimers Disease

Cerebrospinal Fluid Changes in the Renin-Angiotensin System in Alzheimer’s Disease Pubmed: 31594235

Andrologia

Seminal levels of angiotensin II and angiotensin II type 2 receptor expression on spermatozoa in varicocele patients: Relation to fertility status Pubmed: 31989676

Molecular Medicine Reports

BPA disrupts 17‑estradiol‑mediated hepatic protection against ischemia/reperfusion injury in rat liver by upregulating the Ang II/AT1R signaling pathway Pubmed: 32319667

MOLECULAR MEDICINE REPORTS

The sympathetic transmitter norepinephrine inhibits VSMC proliferation induced by TGFβ by suppressing the expression of the TGFβ receptor ALK5 in aorta … Pubmed: 32319652

Cell Mol Neurobiol

Predator Scent-Induced Sensitization of Hypertension and Anxiety-like Behaviors Pubmed: 33201417

biochemistry biophysical reports

Effect of repetitive potassium iodide on thyroid and cardiovascular functions in elderly rats Pubmed: 33024842

bioscience reports

Angiotensin II enhances the proliferation of Natural Killer/T-cell lymphoma cells via activating PI3K/Akt signaling pathway Pubmed: 32969473

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY

A (pro) renin receptor decoy peptide PRO20 protects against adriamycin-induced nephropathy by targeting intrarenal renin-angiotensin system Pubmed: 32865014

Acta Endocrinologica-Bucharest

THE ROLE OF HIGH FOLLICULAR LEVELS OF ANGIOTENSIN II AND VASCULAR ENDOTHELIAL GROWTH FACTOR IN ANTICIPATING THE … Pubmed: 32685035

JOURNAL OF PROTEOME RESEARCH

Proteomics and informatics for understanding phases and identifying biomarkers in COVID-19 disease Pubmed: 32657586

Scientific Reports

Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in rats Pubmed: 32616735

BIOMEDICINE & PHARMACOTHERAPY

Semen Brassicae reduces thoracic aortic remodeling, inflammation, and oxidative damage in spontaneously hypertensive rats Pubmed: 32570115

CLINICAL AND EXPERIMENTAL HYPERTENSION

Hyperinsulinemia rather than insulin resistance itself induces blood pressure elevation in high fat diet-fed rats Pubmed: 32349626

Evidence-Based Complementary and Alternative Medicine

Tianma Gouteng Decoction Exerts Cardiovascular Protection by Upregulating OPG and TRAIL in Spontaneously Hypertensive Rats Pubmed: 33133215

Oxidative Medicine and Cellular Longevity

Sinapic Acid Attenuates Cardiovascular Disorders in Rats by Modulating Reactive Oxygen Species and Angiotensin Receptor Expression Pubmed: 32765804

Nutrition

Gut Dysbiosis Contributes to High Fructose-Induced Salt Sensitive Hypertension In Sprague-Dawley Rats

 

Dikkat Eksikliği Hiperaktivite Bozukluğu Tanısı Olan Anjiyotensin I, II, III, Alamandin peptid, Anjiyotensin Dönüştürücü Enzim (ACE), NADPH Oksidaz Enzim Serum …

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