Elisa kit for IL-10

Our Elisa Kit products are perfect for the analysis of biological fluids, including Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids.

Our Elisa Kit for IL-10 has high sensitivity and excellent specificity for the detection of Interleukin 10 (IL10). ConductScience offers Elisa Kits for Analysis.

$685.00

Specifications

This assay has high sensitivity and excellent specificity for the detection of Interleukin 10 (IL10).
No significant cross-reactivity or interference between Interleukin 10 (IL10) and analogs was observed.

Recovery

Matrices listed below were spiked with a certain level of recombinant Interleukin 10 (IL10) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 10 (IL10) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)91-10597
EDTA plasma(n=5)78-9995
heparin plasma(n=5)94-10598

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle, and high-level Interleukin 10 (IL10) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle, and high-level Interleukin 10 (IL10) were tested on 3 different plates, with 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 10 (IL10) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)78-99%89-96%85-98%92-102%
EDTA plasma(n=5)86-102%93-101%95-103%99-105%
heparin plasma(n=5)80-101%78-98%78-96%95-105%

Stability

The stability of the kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1×120µLAssay Diluent A1×12mL
Detection Reagent B1×120µLAssay Diluent B1×12mL
TMB Substrate1×9mLStop Solution1×6mL
Wash Buffer (30 × concentrate)1×20mLInstruction manual1

Procedure

1. Prepare all reagents, samples, and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate for 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate for 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate for 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Additional information

Brand

Cloud Clone

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