ELISA Kit for Insulin (INS)
Our Elisa Kit products are perfect for the analysis of biological fluids, including Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids.
This assay has high sensitivity and excellent specificity for the detection of Insulin (INS).
No significant cross-reactivity or interference between Insulin (INS) and analogs was observed.
Matrices listed below were spiked with a certain level of recombinant Insulin (INS) and the recovery rates were calculated by comparing the measured value to the expected amount of Insulin (INS) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle, and high-level Insulin (INS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high-level Insulin (INS) were tested on 3 different plates, with 8 replicates on each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentrations of Insulin (INS) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of the kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.
To minimize extra influence on the performance, operation procedures, and lab conditions, especially room temperature, air humidity, and incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
- Prepare all reagents, samples, and standards;
- Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately.
- Shake and mix. Incubate 1 hour at 37°C
- Aspirate and wash 3 times;
- Add 100µL prepared Detection Reagent B. Incubate for 30 minutes at 37°C;
- Aspirate and wash 5 times;
- Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
- Add 50µL Stop Solution. Read at 450 nm immediately.