Elisa Kit for Melatonin
Our Elisa Kit products are perfect for the analysis of biological fluids, including Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids.
Elisa Kit for Melatonin has high sensitivity and excellent specificity for the detection of Melatonin (MT). ConductScience offers Elisa Kits for Analysis.
Specifications
This assay has high sensitivity and excellent specificity for the detection of Melatonin (MT).
No significant cross-reactivity or interference between Melatonin (MT) and analogs was observed.
Recovery
Matrices listed below were spiked with a certain level of Melatonin (MT) and the recovery rates were calculated by comparing the measured value to the expected amount of Melatonin (MT) in samples.
| Matrix | Recovery range (%) | Average(%) |
|---|---|---|
| serum(n=5) | 86-101 | 96 |
| EDTA plasma(n=5) | 85-101 | 93 |
| heparin plasma(n=5) | 87-94 | 90 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle, and high-level Melatonin (MT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high-level Melatonin (MT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Melatonin (MT) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 83-93% | 80-93% | 93-101% | 96-103% |
| EDTA plasma(n=5) | 79-91% | 78-96% | 79-94% | 99-105% |
| heparin plasma(n=5) | 81-103% | 82-99% | 80-101% | 87-99% |
Stability
The stability of the kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
|---|---|---|---|
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1 | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| Reagent Diluent | 1×300µL | Stop Solution | 1×6mL |
| TMB Substrate | 1×9mL | Instruction manual | 1 |
| Wash Buffer (30 × concentrate) | 1×20mL |
Procedure
1. Prepare all reagents, samples, and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate for 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate for 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
Additional information
| Brand | Cloud Clone |
|---|
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