ELISA Kit for Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a)
Our Elisa Kit products are perfect for the analysis of biological fluids, including Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids.
Specificity
This assay has high sensitivity and excellent specificity for the detection of Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a).
No significant cross-reactivity or interference between Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) and analogs was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle, and high-level Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle, and high-level Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) were tested on 3 different plates, with 8 replicates on each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-assay: CV<12%
Stability
The stability of the kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.
To minimize extra influence on the performance, operation procedures, and lab conditions, especially room temperature, air humidity, and incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
|---|---|---|---|
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
- Prepare all reagents, samples, and standards;
- Add 100µL standard or sample to each well. Incubate 1 hour at 37°C;
- Aspirate and add 100µL prepared Detection Reagent A. Incubate for 1 hour at 37°C;
- Aspirate and wash 3 times;
- Add 100µL prepared Detection Reagent B. Incubate for 30 minutes at 37°C;
- Aspirate and wash 5 times;
- Add 90µL Substrate Solution. Incubate for 10-20 minutes at 37°C;
- Add 50µL Stop Solution. Read at 450nm immediately.
Additional information
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