ELISA Kit for Thymosin Beta 4 (TMSB4X)
Our Elisa products are perfect for the analysis of biological fluids, including Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids.
Please enquire about species-specific products and we can help find the right match for you.
ConductScience offers the Mini Samples Thymosin Beta 4 (TMSB4X). Our Elisa products are perfect for the analysis of biological fluids, including Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids.
Test Method | Organism species | Detection range | Sensitivity |
|---|---|---|---|
Competitive Inhibition | Mus musculus (Mouse) | 9.88-800ng/mL | <3.59pg/mL. |
Sensitivity
This assay has high sensitivity and excellent specificity for the detection of Mini Samples Parathyroid Hormone (PTH).
No significant cross-reactivity or interference between Mini Samples Parathyroid Hormone (PTH) and analogs was observed.
Recovery
Matrices listed below were spiked with a certain level of recombinant Mini Samples Parathyroid Hormone (PTH) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Parathyroid Hormone (PTH) in samples.
| Matrix | Recovery range (%) | Average(%) |
|---|---|---|
| serum(n=5) | 97-105 | 101 |
| EDTA plasma(n=5) | 84-95 | 91 |
| heparin plasma(n=5) | 93-105 | 102 |
Stability
The stability of the kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.
To minimize extra influence on the performance, operation procedures, and lab conditions, especially room temperature, air humidity, and incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
|---|---|---|---|
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | $4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×60µL | Assay Diluent A | 1×6mL |
| Detection Reagent B | 1×60µL | Assay Diluent B | 1×6mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×3mL |
| Wash Buffer (30 × concentrate) | 1×10mL | Instruction manual | 1 |
Assay procedure summary
- Prepare all reagents, samples, and standards;
- Add 25µL standard or sample to each well.
- Aspirate and wash 3 times;
- Add 50µL prepared Detection Reagent B. Incubate for 30 minutes at 37°C;
- Aspirate and wash 5 times;
- Add 50µL Substrate Solution. Incubate for 10-20 minutes at 37°C;
- Add 25µL Stop Solution. Read at 450 nm immediately.
It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. Mini Samples ELISA Kits are available in the following species:
Pan-species
Multi-species
Human
Simian
Mouse
Rat
Canine
Porcine
Bovine
Rabbit
Chicken
Sheep
Please enquire about species-specific products and we can help find the right match for you.
Documentation
What is an ELISA (enzyme-linked immunosorbent assay)?
This plate-based assay is used to detect and quantify soluble macro-molecules “the target antigen” such as peptides, proteins, antibodies, and hormones. Antibodies specific to the target antigen are immobilized on a microplate surface which is complexed with an antibody connected to a reporter enzyme. Detection of the target antigen is made by assessment of reporter enzyme activity during incubation. The target antigen binds to the immobilized antibody linked to an appropriate substrate to produce a product to measure the amount of target antigen bound to the plate.
Competitive inhibition
The concentration of target antigen is determined by signal interference as the target antigen and labeled reference sample compete to bind to the immobilized antibody.
Double-antibody sandwich
This method is commonly used to detect the concentration of an unknown target antigen in a sample. Plates are coated with an immobilized antibody and a blocking buffer is applied to reduce non-specific binding. The sample containing the target antigen is applied to the plate and binds to the immobilized antibodies. Once the plate is washed, a detection antibody solution is added which binds a different epitope on the target antigen to the immobilized antibody. A chromogenic, chemiluminescent, or fluorescent detection substrate is added to measure the amount of target antigen present in the sample relative to the amount of pigment, light, or fluorescent signal produced from the detection substrate.
Mini-Elisa Kits
A compact version of the Elisa Kits
Clia (Chemiluminescence) Kit
A combination of Chemiluminescence and immunohistochemical reaction techniques, CLIA techniques utilize light-emitting chemical probes to measure the chemical reaction between target antigens and kit antibodies.
Detection range
The range of target antigen the assay is able to detect.
Sensitivity
This is the lowest value of the target antigen in the assay buffer that the assay can statistically differentiate from background “noise”.
Recovery Range
With a known amount of target antigen added (spiked), the Elisa is run to assess the recovery of the target antigen compared to an identical spiked standard diluent.
Precision
The closeness of agreement between independent test results obtained under stipulated conditions.
- Intra-assay precision: Reproducibility between wells within an assay plate to ensure samples running in different wells give comparable results.
- Inter-assay precision: Reproducibility between assays performed on separate occasions. This is typically less than <10% to ensure consistent results over time and between different kits.
Linearity
Using serial dilutions of a known amount of target antigen (spiked), the precision of results over a range of dilutions is assessed to ensure the assay works with different concentrations of the target antigen.
Additional information
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