Elisa Kit for TNF-a (1,000pg/mL)

Category: SKU: CC-EK-TNF-A

Our Elisa Kit products are perfect for the analysis of biological fluids, including Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids.

ConductScience offers Elisa Kits for Analysis.


This assay has high sensitivity and excellent specificity for the detection of Tumor Necrosis Factor Alpha (TNFa).
No significant cross-reactivity or interference between Tumor Necrosis Factor Alpha (TNFa) and analogs was observed.


Matrices listed below were spiked with a certain level of recombinant Tumor Necrosis Factor Alpha (TNFa). The recovery rates were calculated by comparing the measured value to the expected amount of Tumor Necrosis Factor Alpha (TNFa) in samples.

MatrixRecovery range (%)Average(%)
EDTA plasma(n=5)81-9288
heparin plasma(n=5)82-9888

Intra-assay Precision (Precision within an assay): 3 samples with low, middle, and high-level Tumor Necrosis Factor Alpha (TNFa) were tested 20 times on one plate, respectively. 
Inter-assay Precision (Precision between assays): 3 samples with low, middle, and high-level Tumor Necrosis Factor Alpha (TNFa) were tested on 3 different plates, 8 replicates in each plate. 
CV(%) = SD/meanX100 
Intra-Assay: CV<10% 
Inter-assay: CV<12% 


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tumor Necrosis Factor Alpha (TNFa) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

EDTA plasma(n=5)84-95%81-102%84-94%78-103%
heparin plasma(n=5)95-102%98-105%82-101%86-95%

The loss rate of activity determines the stability of the kit. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. 
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Packaging & Shipping
Reagents and materials provided
Pre-coated, ready-to-use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1×120µLAssay Diluent A1×12mL
Detection Reagent B1×120µLAssay Diluent B1×12mL
TMB Substrate1×9mLStop Solution1×6mL
Wash Buffer (30 × concentrates)1×20mLInstruction manual1

1. Prepare all reagents, samples, and standards;
2. Add 100µL standard or sample to each well. Incubate for 1 hour at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate for 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate for 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate for 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately. 

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Elisa Kit for TNF-a (1,000pg/mL)
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