$326.70 – $1,190.50
Euthanasia Chambers are simple chambers quick and easy to use made of complete acrylic plastic for humane euthanasia of test subjects. Our euthanasia chambers include the container, lid, locking clamp and inlet & outlet with stopcock are all 5/16″ barbed fittings fit for standard CO2 gas tubes. We offer euthanasia chamber for a range of species in different sizes
Model
| Species
| Dimensions (cm)
| Volume (L)
|
ORCH-EUTH1A
| Mice
| 12x10x12
| 1.43
|
ORCH-EUTH1B
| Rat (Up to 300g)
| 23x11x17
| 4.29
|
ORCH-EUTH2A
| Mice and Rats (Up to 500g)
| 27.94×14.60×17.78
| 7.25
|
ORCH-EUTH2A-2
| Rat, Guinea Pig (Up to 350g) Two Compartments.
| 55x18x18 Each compartment dimensions: 27x18x18
| 17.78
|
ORCH-EUTH2A-4
| Rat, Guinea Pig up to 350g Four Compartments.
| 108x17x18 Each compartment dimensions: 27x17x18cm
| 33.00
|
ORCH-EUTH3
| Rat, Guinea Pig (Up to 350g)
| 40.64×20.32×20.32
| 16.78
|
ORCH-EUTH4
| Rabbit (up to 2000g)
| 50.8 x 30.48 x 38.10
| 58.99
|
ORCH-EUTH5
| Rabbit (up to 4000g)
| 71.12 x 38.10 x 38.10
| 103.23
|
ORCH-EUTH6
| Guinea Pig
| 101.6 x 50.8 x 20.32
| 104.87
|
Euthanasia is an act of causing a humane death in an animal by triggering speedy loss of consciousness, which leads to death with the least amount of pain, discomfort, or distress possible. An acceptable method of euthanasia, as per the American Veterinary Medical Association, makes an animal unconscious and dismissive to pain and distress as rapidly as possible, followed by the dysfunctioning of all respiratory and circulatory functions, as well as brain activity. The Euthanasia chamber euthanizes laboratory rodents through CO2 inhalation. It contains barbed fittings that can fit standard CO2 gas tubes
.
There are several methods for euthanizing animals; however, an ideal euthanasia procedure fits certain characteristics for both the animal, employer, and the chemical used. Ideally, it should ensure the detention of an animal with minimal distress and the induction of sudden and permanent insensibility with minimal distress to the animal. Moreover, this technique should be acceptable to both the public and the person performing the treatment. It should also be cost-effective and easily performed by non-veterinary staff without substantial training. Finally, the chemical used for euthanizing is also critical. It should pose no or minimal hazard to the person doing the procedure, and leave no damage or residues that would hinder investigation or future use of the bodies. It should also not produce any residues that threaten other animals after the dead bodies are disposed of.
Considering all the parameters mentioned above for the best method of euthanasia, inhalation of CO2 is the closest to that ideal. It reliably and quickly produces unconsciousness with minimal distress to the animal. Moreover, it has no significant safety risks (Boivin et. al, 2017)
The CO2 gas is introduced into the chamber and slowly makes the animal lose consciousness, leading to a slow and humane death.
The most common form of euthanasia utilized for small animals is carbon dioxide (CO2) inhalation. Although CO2 is typically considered an appropriate euthanasia agent for small animals when provided properly, its acceptability is contingent on several essential parameters. The animals must be put to sleep by skilled workers. During euthanasia, different species should not be intermingled. When possible, animals should be euthanized individually. However, grouping animals for euthanasia is another approved and regular practice. Make sure the chamber is not too crowded. Euthanasia should be avoided in the animal room wherever possible.
The rate at which CO2 is displaced is crucial for humane CO2 application. Hence, a pressure-reducing regulator, flow meter, or restriction valve must be employed. A two-stage regulator provides the most control overflow rates. An initial regulator reduces the pressure in the tank to a predefined level, and then a flowmeter, flow gauge, or restriction valve delivers a precise CO2 flow to the euthanasia chamber. The flow rate can be increased after the animals are unconscious to reduce the time to death. An unconscious animal is defined as the loss of the righting reflex or achievement of lateral recumbency in the animal (Shomer et al., 2020).
The following equation determines the volume of the euthanasia chamber:
Euthanasia chamber volume in liters = (Height in cm) × (Width in cm) × (Length in cm) /1000
The following assessment criteria apply to all species and euthanasia procedures.
Heart rate
The heart rate must be monitored for at least five minutes. Direct palpation of the carotid or femoral artery pulses and direct heart palpation are the most accurate methods of diagnosis. ECG can also be used to confirm death if there is no electrical activity in the heart.
Light-induced pupillary response
This should be done by shining a bright light into the animal’s eyes. A neurological response is shown by pupil constriction. The pupils will become dilated and insensitive to light after death. Some medicines and experimental substances, e.g., anticholinergics like atropine, also can inhibit pupillary responsiveness or modify it in other ways.
Deeply sedated animals can have shallow and erratic breathing patterns, which might be mistaken for a lack of spontaneous respiration. As a result, the absence of spontaneous breathing should not be utilized as the sole criterion for euthanasia confirmation.
Euthanasia is a critical research procedure. Several times scientists have to find out the effects of respiratory distress or hypoxia on the whole body in normal, diseased, or specific conditions such as injury, pregnancy, etc. In those cases, the euthanasia chamber helps establish hypoxic conditions for lab animals in a controlled environment with minimal pain. For example, Hallak et al. (2000) researched the damaging effects of maternal hypoxia on the fetal neurons and CNS. They utilized a euthanasia chamber as a hypoxia chamber and reported the significant neuronal damage with overall reduced brain and body size of the fetus. Similarly, Lauder et al. (2011) reported the reduced intraperitoneal adhesions with chitosan-dextran gel. For the observation of the results, they euthanized the rats using a euthanasia chamber.
Euthanasia is a sensitive process. It should be led with compassion and sympathy. On the other hand, it demands proper technique and training. Following are some of the suggested precautions:
Strengths:
The Euthanasia chamber is a specially designed instrument for CO2 euthanasia that allows euthanizing small lab animals with minimum pain and distress. It allows easy observation of animals with fully controllable CO2 flow rates.
Limitations
Several studies have shown that CO2 causes pain and discomfort in animals, so its usage should be carefully examined (Conlee et al., 2005).
Species | Guinea pig, Large Rodents (Rabbits up to 2000g), Large Rodents (Rabbits up to 4000g(, Mice, Rat (up to 500g), Mouse, Rat (up to 300g), Rat, Guinea pig (up to 350g) – 2 compartments, Rat, Guinea pig (up to 350g) – 4 compartments |
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