Open Field test is a popular protocol used to assess exploratory behavior and anxiety. Thigmotaxis in the open field is used to evaluate anxiolytic, anxiogenic and even non-pharmacological treatments. Ambulation is the most common behavior studied with this maze, but others such as latency or rearing can also be measured. In addition, objects can be added for a modification similar to the novel object recognition field.
Walls can be both opaque or clear. We recommend that clear walls are used for measurements of anxiety, while opaque walls are used if assessments include novel object recognition.
The Zebrafish T maze is an enclosed apparatus in the form of a T placed horizontally, similar to the Y maze. Animals usually start from the base (long arm) of the T and allowed to choose one of the goal arms. The test relies on either spontaneous alternation or rewarded alternation(1). See the video for how to grade this maze. Optional food wells, doors, and return to start arms can be added to your order a-la-carte. Non-reflective options are made in the interior of the maze on request.