Flat Bottom Restrainers are manufactured from perspex cylinders mounted on a flat black perspex base. The restrainers give access through both extremes, by opening the respective tilting door that is fixed by a screw at the upper part of the restrainer. Rodents can be immobilised by sliding the doors along the restrainer. A slot in the base of the door allows for the whole tail to be available for the pulse transducer and cuff installation, the i.v. drug administration or blood extraction.
The easy operation of the doors and its disposition on the extremes of the restrainer makes the manipulation of the animal convenient and avoids the animal to be dragged backwards, protecting it from injuries.
RFLSI Pro Laser Speckle Perfusion Imager is based on the latest LSCI technology (Laser Speckle Contrast Imaging/LSCI, Laser Speckle Ratio Analysis). With the advantages of the unique non-contact, high time and spatial resolution, rapid imaging, it provides a new recording way for the life sciences basic research and clinical medical, which can achieve real-time dynamic blood flow monitoring and video imaging, it is the vital basis for understanding the organization, organ pathology and physiological indicators. The instrument does not need any contrast agent, the time resolution can be in milliseconds, the spatial resolution can be in microns. It also achieves the requirements of real-time observation of microvascular blood flow distribution and relative changes in values.
Spatial resolution of the blood flow imaging: 3.3?m/pixel- 2?m/pixel
Laser type: laser diode, wavelengths 785nm
Monitoring camera power 90mW
Low noise 12bit fast camera for stable flow-rate measurement
Monitoring distance: 110mm
Real-time display of diameter changes and angle
Effective pixels:1800000 pixels /cm2; monitoring pixel is 656*494 pixels
Continuous recording and interval recording modes
Image monitoring area is 14.4 x10.9 mm -2.15 x1.62mm
imaging speed is not less than 60 fps in full amplitude state
ROI area and vessel diameter measurements can be added arbitrarily during the recording process or off-line analysis to support any shape and number of ROI choices
Optical magnification: conventional 0.67-4.5 times, 0.071NA, optional 0.5 times, 2 times auxiliary objective lens
increase the auxiliary objective lens. Monitoring area and blood flow imaging spatial resolution can be adjusted accordingly
The monitoring records can be exported to AVI format video files, including curves, blood flow, experimental process records
Output video can be adjusted as required
Microsurgery is performed under magnification employing advanced diploscopes, specialized precision tools, and various operating procedures, based on delicate manipulation of microstructures that require highly precise and reliable instruments.
The microsurgical methods are primarily used for vascular anastomosis, nerve coaptation, tissue transplantation, and reattachment of the amputated body parts of the rodents.