Product Description
The MicroQuant Cuvette is a high-quality, UV-transparent plastic cuvette designed for the accurate and precise measurement of small sample volumes. With a measurement range of 0.5 – 5 µL and a path length of 1 mm, this cuvette is ideal for use in spectrophotometry applications, particularly in the fields of molecular biology, biochemistry, and analytical chemistry. The cuvette is compatible with most UV-Vis spectrophotometers and offers an accuracy of ±1% at 260 nm.
Product Application
The MicroQuant Cuvette is a versatile tool for a wide range of applications in research, clinical diagnostics, and biotechnology. It can be used for the quantification of nucleic acids, proteins, and enzymes, as well as for the measurement of the concentration of analytes in drug discovery and environmental monitoring. The cuvette is also suitable for microbial growth assays, bacterial quantification, and cell viability assays.
Protocol
- Load the sample into the cuvette using a pipette. Ensure that the sample volume falls within the measurement range of 0.5 – 5 µL.
- Insert the cuvette into the spectrophotometer and record the absorbance at the desired wavelength.
- Clean the cuvette with a suitable laboratory cleaning solution before reuse.
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Strengths and Limitations
One of the major strengths of the MicroQuant Cuvette is its high precision and accuracy, allowing for the measurement of small sample volumes with a high degree of confidence. The cuvette is also reusable, reducing the cost of experimentation and promoting sustainability. However, one limitation is that the cuvette can only accommodate a small sample volume, which may not be suitable for some applications that require larger sample volumes.
Summary
The MicroQuant Cuvette is a reliable and efficient tool for the measurement of small sample volumes in spectrophotometry applications. With high precision and accuracy, the cuvette is widely used in molecular biology, biochemistry, and analytical chemistry research. Its reusable nature makes it a cost-effective and environmentally friendly option.
References
- Turcotte, R., & Rainville, S. (2012). Determination of DNA concentration and purity using the Quantitate Micro-Volume Cuvette. Bio-Rad Bulletin, 6408.