The ConductScience Microvolume Spectrophotometer is a reliable and compact instrument designed to precisely measure nucleic acid and protein samples. This dual-function spectrophotometer offers flexibility for various applications with a built-in 0.5mm pathlength micro-volume port and a 10mm pathlength cuvette holder. The high-resolution color touchscreen and user-friendly software interface make sample measurement and analysis a breeze. The ConductScience Microvolume Spectrophotometer delivers accurate and reproducible results, making it an essential tool for any molecular biology laboratory.
|Light source:||Xenon flash lamp|
|Detector:||2048-element linear CCD array|
|Path length:||0.5 mm and 1.0 mm for microvolume measurement, 10 mm for standard cuvette measurements|
|Volume range:||0.5 µL to 10 µL for microvolume measurements, 0.5 mL to 2 mL for standard cuvette measurements|
|Concentration range:||2 ng/µL to 15,000 ng/µL for dsDNA, 0.1 ng/µL to 2000 ng/µL for RNA, 0.03 mg/mL to 200 mg/mL for protein|
|Measurement time:||<5 seconds for microvolume measurements, <10 seconds for standard cuvette measurements|
|Measurement modes:||microvolume, standard cuvette, kinetics, OD600 cell density measurement, and custom methods|
|Software:||Touchscreen interface with pre-loaded methods and user-defined custom methods, optional PC software for data export and further analysis|
|Connectivity:||USB and Ethernet ports for data transfer and software updates|
|Dimensions:||20.5 cm x 25 cm x 16 cm (W x D x H)|
|Power supply:||100-240 V AC, 50/60 Hz|
|Warranty:||1 year limited warranty|
A microvolume spectrophotometer is a laboratory instrument used to measure the concentration of nucleic acids, proteins, and other biomolecules in small sample volumes. The Microvolume Spectrophotometer is a compact, easy-to-use instrument that allows accurate and reliable measurements of samples as small as 0.5 µl.
Traditional spectrophotometers require relatively large sample volumes, which can be problematic when working with precious or limited samples. Microvolume spectrophotometers have emerged as a solution to this problem, enabling researchers to measure small sample volumes with high accuracy and sensitivity.
Apparatus and equipment
The Microvolume Spectrophotometer is a stand-alone instrument that requires no additional software or computer connections. It has a built-in touchscreen interface for easy operation, and a range of measurement modes for measuring absorbance, concentration, purity, and more. The instrument is compatible with a range of sample types, including DNA, RNA, proteins, and microarrays.
- Turn on the instrument and allow it to warm up for at least 10 minutes.
- Clean the sampling surface with a lint-free cloth or cotton swab.
- Ensure that the instrument is set to the appropriate wavelength for your sample.
- Place a drop of your sample onto the sampling surface.
- Close the lid gently to avoid damaging the sampling surface or the drop.
- Select the measurement mode (DNA, RNA, protein, or general mode) based on your sample type and concentration.
- Enter the appropriate sample ID and concentration units (ng/μL or μg/mL).
- Click “Measure” to initiate the measurement process.
- Record the results, and repeat the process for multiple samples if necessary.
- Clean the sampling surface with a lint-free cloth or cotton swab after each use.
- Ensure that the sampling surface is clean and free of debris before each measurement to avoid inaccurate readings.
- Microvolume spectrophotometers are designed to measure small sample volumes (1-2 μL) and may not be suitable for larger samples.
Microvolume spectrophotometers have become increasingly popular in recent years, particularly in the fields of genomics and proteomics. They offer several advantages over traditional spectrophotometers, including the ability to measure small sample volumes, which reduces the risk of sample degradation, and the ability to measure multiple samples in a short amount of time. Several studies have also shown that microvolume spectrophotometers can provide more accurate and reliable measurements than traditional spectrophotometers.
Microvolume spectrophotometer offers several strengths, including their compact size, easy-to-use interface, and high accuracy and sensitivity. The instrument is also compatible with a range of sample types, making it a versatile instrument for a wide range of applications.
One limitation of the microvolume spectrophotometer is its sample volume range, which is limited to 0.5 µl to 10 µl. This may be problematic for researchers working with extremely small sample volumes or for those who require higher sample throughput. Additionally, the instrument’s touchscreen interface may not be ideal for all users, particularly those who prefer a traditional button-based interface.
The Microvolume Spectrophotometer is a compact, easy-to-use instrument that offers high accuracy and sensitivity for measuring nucleic acids, proteins, and other biomolecules in small sample volumes. While it has some limitations, it is a versatile instrument that is suitable for a wide range of applications in genomics and proteomics.
- Cao Y, Liu Y, Chen L, et al. Comparison of a microvolume spectrophotometer and a conventional spectrophotometer for DNA quantification and purity analysis. J Zhejiang Univ Sci B. 2012;13(2):94-100. doi:10.1631/jzus.B1100140
- McHugh CA, Chen HH, Chow BY, et al. A microfluidic platform for the high-throughput screening of transfection reagents. Lab Chip. 2014;14(15):2823-2830. doi:10.1039/c4lc00146
Analytical Instruments, Cell culture studies, General UV-Vis spectroscopy, Laboratory Equipment, Nucleic Acid quantification, Protein analysis
|Field of study||
Agriculture, Biochemistry, Biotechnology, Food Science, Molecular Biology, Pharmaceuticals
Questions & Answers
There are no questions yet. Be the first to ask a question about this product.