Mini Samples ELISA Kit for Complement Component 5a (C5a)

Our Elisa products are perfect for the analysis of biological fluids, including Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids.

Please enquire about species-specific products and we can help find the right match for you.

$960.00

ConductScience offers the Mini Samples ELISA Kit for Complement Component 5a (C5a). Our Elisa products are perfect for the analysis of biological fluids, including Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids. 

Test Method

Organism speciesDetection rangeSensitivity

Dounle-antibody Sandwich

Mus musculus (Mouse)

1.56-100ng/mL

<0.59ng/mL.

Specificity

This assay has high sensitivity and excellent specificity for the detection of Mini Samples Complement Component 5a (C5a).

No significant cross-reactivity or interference between Mini Samples Complement Component 5a (C5a) and analogs was observed.

Recovery

Matrices listed below were spiked with a certain level of recombinant Mini Samples Complement Component 5a (C5a) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Complement Component 5a (C5a) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)80-9887
EDTA plasma(n=5)94-10298
heparin plasma(n=5)94-10298
Stability

The stability of the kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.

To minimize extra influence on the performance, operation procedures, and lab conditions, especially room temperature, air humidity, and incubator temperature should be strictly controlled.

It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided
ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1×60µLAssay Diluent A1×6mL
Detection Reagent B1×60µLAssay Diluent B1×6mL
TMB Substrate1×4.5mLStop Solution1×3mL
Wash Buffer (30 × concentrate)1×10mLInstruction manual1
Assay procedure summary
  1.  Prepare all reagents, samples, and standards;
  2. Add 25µL standard or sample to each well. Incubate for 1 hour at 37°C;
  3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
  4. Aspirate and wash 3 times;
  5.  Add 25µL prepared Detection Reagent B. Incubate for 30 minutes at 37°C;
  6. Aspirate and wash 5 times;
  7. Add 25µL Substrate Solution. Incubate for 10-20 minutes at 37°C;
  8. Add 20µL Stop Solution. Read at 450nm immediately.

It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.  Mini Samples ELISA Kits are available in the following species:

  • Pan-species

  • Multi-species

  • Human

  • Simian

  • Mouse

  • Rat

  • Canine

  • Porcine

  • Bovine

  • Rabbit 

  • Chicken

  • Sheep

Please enquire about species-specific products and we can help find the right match for you.

Additional information

Brand

Cloud Clone

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