Mini Samples ELISA Kit for Cross Linked C-Telopeptide Of Type I Collagen (CTXI)

Our Elisa products are perfect for the analysis of biological fluids, including Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids.

Please enquire about species-specific products and we can help find the right match for you.

$1,090.00

Description

ConductScience offers the Mini Samples ELISA Kit forCross Linked C-Telopeptide Of Type I Collagen (CTXI). Our Elisa products are perfect for the analysis of biological fluids, including Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids. 

Test Method

Organism speciesDetection rangeSensitivity

Competitive Inhibition

Mus musculus (Mouse)

123.5-10,000pg/mL

<51.5pg/mL.

Specificity

This assay has high sensitivity and excellent specificity for detection of Mini Samples Cross Linked C-Telopeptide Of Type I Collagen (CTXI).

No significant cross-reactivity or interference between Mini Samples Cross Linked C-Telopeptide Of Type I Collagen (CTXI) and analogs was observed.

Recovery

Matrices listed below were spiked with a certain level of recombinant Mini Samples Cross Linked C-Telopeptide Of Type I Collagen (CTXI) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Cross Linked C-Telopeptide Of Type I Collagen (CTXI) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)96-105102
EDTA plasma(n=5)81-10486
heparin plasma(n=5)78-9186
Stability

The stability of the kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.

To minimize extra influence on the performance, operation procedures, and lab conditions, especially room temperature, air humidity, and incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided
ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1×60µLAssay Diluent A1×6mL
Detection Reagent B1×60µLAssay Diluent B1×6mL
TMB Substrate1×9mLStop Solution1×3mL
Wash Buffer (30 × concentrate)1×10mLInstruction manual1
Assay procedure summary
  1.  Prepare all reagents, samples and standards;
  2. Add 25µL standard or sample to each well.
  3. Aspirate and wash 3 times;
  4. Add 50µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
  5. Aspirate and wash 5 times;
  6. Add 50µL Substrate Solution. Incubate 10-20 minutes at 37°C;
  7. Add 25µL Stop Solution. Read at 450 nm immediately.

It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.  Mini Samples ELISA Kits are available in the following species:

  • Pan-species

  • Multi-species

  • Human

  • Simian

  • Mouse

  • Rat

  • Canine

  • Porcine

  • Bovine

  • Rabbit 

  • Chicken

  • Sheep

Please enquire about species-specific products and we can help find the right match for you.

Additional information

Brand

Cloud Clone

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