ConductScience offers the Mini Samples ELISA Kit for Laminin (LN). Our Elisa products are perfect for the analysis of biological fluids, including Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids.
|Organism species||Detection range||Sensitivity|
|Double-antibody Sandwich||Mus musculus (Mouse)||0.156-10ng/mL|
This assay has high sensitivity and excellent specificity for the detection of Mini Samples Laminin (LN).
No significant cross-reactivity or interference between Mini Samples Laminin (LN) and analogs was observed.
Matrices listed below were spiked with a certain level of recombinant Mini Samples Laminin (LN) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Laminin (LN) in samples.
|Matrix||Recovery range (%)||Average(%)|
The stability of the kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.
To minimize extra influence on the performance, operation procedures, and lab conditions, especially room temperature, air humidity, and incubator temperature should be strictly controlled.
It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×60µL||Assay Diluent A||1×6mL|
|Detection Reagent B||1×60µL||Assay Diluent B||1×6mL|
|TMB Substrate||1×4.5mL||Stop Solution||1×3mL|
|Wash Buffer (30 × concentrate)||1×10mL||Instruction manual||1|
Assay procedure summary
- . Prepare all reagents, samples, and standards;
- Add 25µL standard or sample to each well. Incubate for 1 hour at 37°C;
- Aspirate and add 25µL prepared Detection Reagent A. Incubate for 1 hour at 37°C;
- Aspirate and wash 3 times;
- Add 25µL prepared Detection Reagent B. Incubate for 30 minutes at 37°C;
- Aspirate and wash 5 times;
- Add 25µL Substrate Solution. Incubate for 10-20 minutes at 37°C;
- Add 20µL Stop Solution. Read at 450nm immediately.
It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. Mini Samples ELISA Kits are available in the following species:
Please enquire about species-specific products and we can help find the right match for you.