ConductScience offers the Mini Samples ELISA Kit for Osteocalcin (OC). Our Elisa products are perfect for the analysis of biological fluids, including Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids.Â
Test Method | Organism species | Detection range | Sensitivity | Manual |
|---|---|---|---|---|
| Double-antibody Sandwich | Mus musculus (Mouse) | 0.156-10ng/mL | <0.057ng/mL. |
Sensitivity
This assay has high sensitivity with no significant cross-reactivity or interference between this reagent and analogs.
Recovery
Matrices listed below were spiked with a certain level of recombinant Mini Samples Osteocalcin (OC) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Osteocalcin (OC) in samples.
| Matrix | Recovery range (%) | Average(%) |
|---|---|---|
| serum(n=5) | 82-94 | 91 |
| EDTA plasma(n=5) | 85-99 | 88 |
| heparin plasma(n=5) | 91-98 | 94 |
Stability
The activity loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions (see manual for storage advice).
To minimize extra influence on the performance, operation procedures, and lab conditions, especially room temperature, air humidity, and incubator temperature should be strictly controlled.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
|---|---|---|---|
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×60µL | Assay Diluent A | 1×6mL |
| Detection Reagent B | 1×60µL | Assay Diluent B | 1×6mL |
| TMB Substrate | 1×4.5mL | Stop Solution | 1×3mL |
| Wash Buffer (30 × concentrate) | 1×10mL | Instruction manual | 1 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Osteocalcin (OC) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Reagents | Quantity | Reagents | Quantity |
|---|---|---|---|
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×60µL | Assay Diluent A | 1×6mL |
| Detection Reagent B | 1×60µL | Assay Diluent B | 1×6mL |
| TMB Substrate | 1×4.5mL | Stop Solution | 1×3mL |
| Wash Buffer (30 × concentrate) | 1×10mL | Instruction manual | 1 |
Assay procedure summary
- Prepare all reagents, samples, and standards;
- Add 25µL standard or sample to each well. Incubate for 1 hour at 37°C;
- Aspirate and add 25µL prepared Detection Reagent A. Incubate for 1 hour at 37°C;
- Aspirate and wash 3 times;
- Add 25µL prepared Detection Reagent B. Incubate for 30 minutes at 37°C;
- Aspirate and wash 5 times;
- Add 25µL Substrate Solution. Incubate for 10-20 minutes at 37°C;
- Add 20µL Stop Solution. Read at 450nm immediately.
It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. Mini Samples ELISA Kits are available in the following species:
Pan-species
Multi-species
Human
Simian
Mouse
Rat
Canine
Porcine
Bovine
RabbitÂ
Chicken
Sheep
Please enquire about species-specific products and we can help find the right match for you.
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| Brand | Cloud Clone |
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