ConductScience offers the Mini Samples ELISA Kit for Parathyroid Hormone (PTH). Our Elisa products are perfect for the analysis of biological fluids, including Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids.
|Organism species||Detection range||Sensitivity|
|Mus musculus (Mouse)|
This assay has high sensitivity and excellent specificity for the detection of Mini Samples Parathyroid Hormone (PTH).
No significant cross-reactivity or interference between Mini Samples Parathyroid Hormone (PTH) and analogs was observed.
Matrices listed below were spiked with a certain level of recombinant Mini Samples Parathyroid Hormone (PTH) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Parathyroid Hormone (PTH) in samples.
|Matrix||Recovery range (%)||Average(%)|
The stability of the kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.
To minimize extra influence on the performance, operation procedures, and lab conditions, especially room temperature, air humidity, and incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||$4|
|Detection Reagent A||1×60µL||Assay Diluent A||1×6mL|
|Detection Reagent B||1×60µL||Assay Diluent B||1×6mL|
|TMB Substrate||1×9mL||Stop Solution||1×3mL|
|Wash Buffer (30 × concentrate)||1×10mL||Instruction manual||1|
Assay procedure summary
- Prepare all reagents, samples, and standards;
- Add 25µL standard or sample to each well.
- Aspirate and wash 3 times;
- Add 50µL prepared Detection Reagent B. Incubate for 30 minutes at 37°C;
- Aspirate and wash 5 times;
- Add 50µL Substrate Solution. Incubate for 10-20 minutes at 37°C;
- Add 25µL Stop Solution. Read at 450 nm immediately.
It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. Mini Samples ELISA Kits are available in the following species:
Please enquire about species-specific products and we can help find the right match for you.