Monoclonal package 4

(From cDNA to ascites purification)

• Gene amplification
• Vector construction
• Protein expression and purification
• Immunization and sera collection
• ELISA identification
• Fusion and hybridoma development
• Isotype determination
• Ascites production and purification (2 clones)

• Recombinant vector
• 1mg protein
• 50ul pre-immune sera (1:10dilution)
• 2-5 clones
• 2-5mg purified antibodies (2 clones)
• ELISA result
• Isotype determination result
• Project report