Monoclonal Package 6

(Western guaranteed monoclonal antibodies)

• Peptide design and synthesis(12-22aa,85%)
• Peptide conjugation
• Immunization and sera collection
• ELISA & Western Blot identification
• Fusion and hybridoma development
• Isotype determination
• Ascites production and purification (2 clones)

• 2-3mg peptides
• 50ul pre-immune sera (1:10dilution)
• 2-5 clones
• 2-5mg purified antibodies (2 clones)
• ELISA & Western Blot results
• Isotype determination result
• Project report