The Research Autoclave is designed to meet your sterilization needs while maintaining a safe, fully automatic operation. Simply load items (liquids, media, instruments, glassware, plasticware, and other research laboratory items) into the autoclave, select the desired program using the touch-screen feature, close the door, and press start.
A “Dry Only” option can be selected to add drying time at the end of the cycle. All settings are customizable to meet your needs. In addition, to record settings and transfer them to a computer, simply insert a USB drive.
ConductScience offers the Research Autoclave.
A research autoclave is a sterilizing device used to destroy microorganisms and potential pathogens in research samples and equipment. The samples or instruments to be sterilized are subjected to high pressure saturated steam at 121°C for 15-20 minutes, depending on the size of contents and load. It is usually used in the laboratory to sterilize surgical equipment, instruments, pharmaceutical items, culture media, and other laboratory materials. The equipment can sterilize solids, hollows, liquids, and materials of various shapes and sizes. Research autoclaves vary in size, shape, and functionality. The choice of the appropriate autoclave depends on the experimental needs and laboratory requirements.
The introduction of the research autoclaves is credited to Charles Chamberland, a colleague of Pasteur, who invented the autoclave to meet Pasteur’s need for sterilization that utilized temperatures higher than 100oC. The instrument was developed between 1876 and the 1880s. Chamberland’s work was furthered by Professor Von Bergmann of Berlin (1885) who introduced steam sterilization. After some years, pressurized steam sterilization was developed to kill resistant spores and pathogens. Since then, the research autoclave has become an essential instrument in biomedical science to ensure contamination-free research.
The research autoclave consists of a central chamber box that holds the loaded materials to be sterilized. The instrument is operated by a number of controls to adjust the parameters such as temperature and pressure. A safety valve is constructed on the autoclave to prevent excess pressure build-up. A steam generator is a water heating unit in the chamber to create steam and pressure. Before discharging the wastewater, the water is cooled by the cooling system to prevent damage to the facility’s sewer system. Some autoclaves are also equipped with a vacuum system to remove air before steam injection.
Autoclave makes use of high temperature (121 °C), and high-pressure steam (15 psi) for 30 minutes to ensure decontamination. Microorganisms are killed by dehydrating the cells under severe conditions. Samples and instruments are decontaminated depending on the nature of the load. For dry solid materials, a faster exhaust cycle is achieved. However, the slow exhaust is required for liquid and biological waste to avoid boiling. Indicator tapes validate the effectiveness of autoclaving. These indicators use heat-sensitive chemical markings to show temperature requirements (121 °C). A change in the tape color implies an effective autoclaving, whereas no change indicates incomplete or no decontamination.
1. Wear proper gloves, safety glasses, and a lab coat.
2. Material preparation
4. Set the temperature at 121 °C and maintain the pressure of 15 psi and turn the autoclave ON.
Autoclaves are widely used in biomedical research for sterilization purposes. It has a wide array of applications in research fields including microbiology, plant physiology, virology, and many others. Hospitals and clinics use the autoclaves to sterilize the instruments before reuse. Scientists also sterilize their equipment to decontaminate and achieve purity in chemical reactions or cultures grown for research. Autoclaves are also used to decontaminate the waste materials before discharge. This not only purifies the waste from bacteria and viruses but also softens some materials, such as plastics to decrease the waste volume. In industries, autoclaves are used for particular purposes such as curing composite materials or growing crystals. Research autoclaves offer a wide range of applications to ensure decontamination and purity in experiments and laboratory studies.