Lab Protocols
Instructions for conducting experiments and carrying out scientific procedures.
Explore a curated collection of protocols designed to support laboratory research across neuroscience, molecular biology, analytical chemistry, histology, electrophysiology, and more. Each protocol includes background theory, required materials, clear step-by-step procedures, and practical troubleshooting tips.
Autoclaves and Sterilizers
An autoclave is an efficient and economical instrument used for sterilizing purposes in labs, healthcare, and dental settings. It works on the principle of steam sterilization. This high-pressurized steam is effective in killing microbes, including endospores.
Cellulose Acetate Electrophoresis
Although gel and capillary electrophoresis are more common in modern labs, cellulose acetate electrophoresis remains an important tool in clinical diagnostics due to its simplicity, speed, and adaptability. This guide covers setup, sample application, run conditions, and result interpretation.
Gel Electrophoresis
Gel electrophoresis began as a form of zone electrophoresis and has evolved to support numerous types of molecular separations. This protocol details gel preparation, sample loading, electrophoretic run parameters, staining, imaging, and common troubleshooting steps.
Our Protocols Resources
Zinc Staining Protocol
Introduction The reversible Zinc stain is a widely used staining method for the detection of...
Read MoreWhat Is Optogenetics and How Does It Work?
Reference to this article: ConductScience, What Is Optogenetics and How Does It Work? (2022). doi.org/10.55157/CS20220704...
Read MoreWhat is Autoradiography?
As an Amazon Associate, ConductScience Inc. earns revenue from qualifying purchases Autoradiography is an imaging...
Read MoreWhat Is A Brain Slice? Everything To Know About A Brain Slice
Introduction Today, we all ā biologists or not ā have an idea about how the...
Read MoreWhat Are the Different Types of Nucleic Acids Extraction Methods?
Nucleic acids such as deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) are macromolecules that carry...
Read MoreWestern blotting Protocol
Introduction The Western blot is a biological technique that allows for the specific identification and...
Read MoreWeight Drop Injury Model
Introduction Traumatic brain injury (TBI) is one of the most common brain injuries caused by...
Read MoreVisual Patch Testing: Definition, Mechanism, and Application
What is Visual Patch Testing? Patch tests are one of the popular diagnostic tools used...
Read MoreVibrating Microtomes: What They Are & Their Applications
What are Vibrating Microtomes? Before the tissue slicers, scientists had to manually cut biological materials...
Read MoreUltracentrifugation Basics and Applications
Need Centrifuges for your lab? Click here Introduction Ultracentrifugation is a specialized technique used to...
Read MoreUltimate Guide to Automated Cell Counter: Plus Purchasing Tips
Reference to this article: ConductScience, Ultimate Guide to Automated Cell Counter: Plus Purchasing Tips (2022)....
Read MoreUltimate Guide on Neuronal or Brain Slice Electrophysiology
What is Brain Slice? Electrophysiology refers to studying the electrical properties of biological cells and...
Read MoreTypes of Tissue Slice Techniques for Slice Preparation
Tissue slices are sections of tissue cut from larger pieces or extracted organs and treated...
Read MoreTRICINE-SDS-PAGE Protocol
Introduction and Principle Proteins in the mass range 1ā100 kDa are commonly separated by TricineāSDS-PAGE....
Read MoreThe Spiral Structure of Chromosome
Introduction A Chromosome is an organized package of DNA, located inside the nucleus. The structure...
Read MoreThe Nuclear Overhauser Effect
Introduction The nuclear overhauser effect is defined as a change in the integrated intensity of...
Read MoreThe Cytogenetics of Fungi
As an Amazon Associate, ConductScience Inc. earns revenue from qualifying purchases Introduction Fungi is a...
Read MoreThe Cryostat-Microtome
Need Microtomes for your studies? Click here A Glimpse into the History of a Cryostat...
Read MoreThe ?-radiation and its effects on chromosomes
Introduction and Historical Perspectives Radiations have been extensively used in medical devices to look into...
Read MoreSpin-Spin coupling in NMR
Need NMR Instruments for your lab? Click here The structure of a molecule can be...
Read MoreSouthern Blotting Protocol
Need Laboratory Equipment for your reasearch? Click here Southern blotting is a hybridization technique used...
Read MoreSilver Staining Protocol
Need stains for your experiments? Click here Silver staining is a powerful technique for protein...
Read MoreSDS-Polyacrylamide Gel Electrophoresis at Neutral pH (NuPAGE)
Introduction and Principle The traditional Laemmli system denaturing conditions are stimulated by the Invitrogen NuPAGE...
Read MoreSafranin Staining
Need stains for your experiments? Click here Introduction Safranin is a cationic dye used in...
Read MoreRodent Craniotomy Protocol
Introduction A craniotomy is a surgical procedure used to temporarily open a part of the...
Read MoreQuinolinic acid rat HD model
Introduction Huntingtonās disease (HD) is an inherited autosomal dominant neuropathological disease caused by a mutation...
Read MoreQuantitative Phosphate Protocol
Introduction: Determination of Inorganic Phosphate in Biologic Samples To find out the inorganic phosphate in...
Read MorePurification of Bacteriophage Ī» Arms using Sucrose Density Gradients
Introduction Replacement vectors or substitution vectors are prepared by removing the central filler segment of...
Read MorePulse Chase Analysis
Need Incubators for your lab? Click here Ā As an Amazon Associate Conductscience Inc earns...
Read MoreProtein Staining Methods
Introduction Protein separation and identification is critical for proteome analysis and requires high resolution and...
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