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Laboratory Techniques

BRADFORD Protein Determination Protocol

Need Laboratory Equipment for your reasearch? Click here Introduction and Principle Developed by Marion M. Bradford in 1976, the Bradford assay is a quick protocol,

Laboratory Techniques

Fast protein liquid chromatography (FPLC) Protocol

Introduction Fast protein liquid chromatography (FPLC) is a type of liquid chromatography that provides high resolution by small-diameter stationary phases for protein characterization and separation.
Filtration Chromatography Protocol
Laboratory Techniques

Gel Filtration Chromatography Protocol

Need benchtop equipment for your lab? Click here Introduction Gel-filtration chromatography, also known as size exclusion chromatography, is a versatile technique that permits the separation

Laboratory Techniques

Ion-exchange Chromatography Protocol

Introduction Ion Exchange Chromatography (IEC) is a powerful liquid chromatographic technique used for bioseparation. The separation is done by a reversible interaction between charged molecules

Laboratory Techniques

KJELDAHL Method Protocol

Introduction and Principle The Kjeldahl method was developed by a brewer called Johann Kjeldahl in 1883. The protocol is built on the principle that strong
Protein Quantification Protocols
Laboratory Techniques

Protein Quantification Protocols

Introduction: Quantitative Method The estimation of proteins via the quantitative method is one of the basic requirements in biochemistry. Proteins, from various perspectives, are substantially
Nucleic Acid Quantification Protocols
Laboratory Techniques

Nucleic Acid Quantification Protocols

Introduction: Quantitative Method DNA and RNA quantifications are broadly utilized in biological and biomedical research. Over the last ten years, many technologies have been developed
Filtration Chromatography Protocol
Laboratory Techniques

Quantitative Phosphate Protocol

Introduction: Determination of Inorganic Phosphate in Biologic Samples To find out the inorganic phosphate in a protein-containing liquid biological sample an aqueous reagent comprises an
Laboratory Techniques

TRICINE-SDS-PAGE Protocol

Introduction and Principle Proteins in the mass range 1–100 kDa are commonly separated by Tricine–SDS-PAGE. For the resolution of proteins less than 30 kDa, it
Laboratory Techniques

Western blotting Protocol

Introduction The Western blot is a biological technique that allows for specific identification and characterization of proteins. In this technique, the proteins are assorted based
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