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Laboratory TechniquesProtocols
June 26, 2019

Fast protein liquid chromatography (FPLC) Protocol

Fast protein liquid chromatography (FPLC) is a type of liquid chromatography that provides high resolution by small-diameter stationary phases for protein characterization and separation. The technique features high loading capacity, biocompatible buffer systems, fast flow rates, and stationary phases for common chromatography modes (e.g., gel filtration, ion exchange, reversed phase,…
Filtration Chromatography Protocol
Laboratory TechniquesProtocols
June 26, 2019

Gel Filtration Chromatography Protocol

Gel-filtration chromatography, also known as the size exclusion chromatography, is a versatile technique that permits the separation of proteins and other biological molecules. The gel filtration chromatography separates the proteins solely based on molecular size differences. For this, a porous matrix is used to which the molecules, for steric reasons,…
Laboratory TechniquesProtocols
June 26, 2019

Ion-exchange Chromatography Protocol

Ion Exchange Chromatography (IEC) is a powerful liquid chromatographic technique used for bioseparation. The separation is done by a reversible interaction between charged molecules of the sample with charged ligands attached to a column. The method offers a sizeable sample-handling capacity, powerful resolving ability, broad applicability, moderate cost, and the…
Laboratory TechniquesProtocols
June 26, 2019

KJELDAHL Method Protocol

The Kjeldahl method was developed by a brewer called Johann Kjeldahl in 1883. The protocol is built on the principle that strong acid helps in the digestion of food so that it releases nitrogen which can be determined by a suitable titration technique. By observing the nitrogen concentration of the…
Protein Quantification Protocols
Laboratory TechniquesProtocols
June 26, 2019

Protein Quantification Protocols

The estimation of proteins via the quantitative method is one of the basic requirements in biochemistry. Proteins, from various perspectives, are substantially more complex than nucleic acids. Thus, it has been hard to give laboratory protocols that can be applied to proteins. The precise quantitation of the amount of protein…
SDS-Polyacrylamide Gel Electrophoresis at Neutral pH (NuPAGE)
Laboratory TechniquesProtocols
June 26, 2019

SDS-Polyacrylamide Gel Electrophoresis at Neutral pH (NuPAGE)

The traditional Laemmli system denaturing conditions are stimulated by the Invitrogen NuPAGE SDS-PAGE gel system which is a revolutionary high-performance polyacrylamide gel electrophoresis system. NuPAGE gels use a unique buffer formulation to maintain a neutral operating pH during electrophoresis, which minimizes protein modifications that can result in poor band resolution.…
Nucleic Acid Quantification Protocols
Laboratory TechniquesProtocols
June 25, 2019

Nucleic Acid Quantification Protocols

DNA and RNA quantifications are broadly utilized in biological and biomedical research. Over the last ten years, many technologies have been developed to enable automated and high-throughput analyses. We are going to learn the assays and different methods of how DNA and RNA quantifications are carried out.This procedure has been…
Filtration Chromatography Protocol
Laboratory TechniquesProtocols
June 25, 2019

Quantitative Phosphate Protocol

To find out the inorganic phosphate in a protein-containing liquid biological sample an aqueous reagent comprises an acid. This acid is capable of reacting with the molybdate salt to form molybdic acid for complexation with phosphate to form phosphomolybdate complexes, a molybdate salt, a nonionic surfactant reagent in an amount…
Conduct Science

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