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A Brief on The NMR Spectroscopy of Proteins

Spectroscopy is “the study of investigation and measurement of the absorption and emission of light or electromagnetic radiation by matter as a function of its wavelength and frequency”. In simple terms, it’s the study of the interaction of light with a matter or substance. Depending on the nature of the…

Anjali SinghApril 13, 2021

Laboratory Techniques Protocols Science

The Nuclear Overhauser Effect

Introduction The nuclear overhauser effect is defined as a change in the integrated intensity of one spin when the equilibrium population of another spin is perturbed by saturation or inversion. So, it is the transfer of nuclear spin polarization from one spin-active nucleus to another. However, the nuclei being irradiated…

Anjali SinghApril 5, 2021

Laboratory Techniques Science

Large Scale Extraction of Bacteriophage λ DNA Using Proteinase K

Large-scale extraction of phage DNA involves digestion of viral coat proteins using a strong protease such as proteinase K followed by extraction with chloroform. This method is suitable for small-scale preparations as well. In the experiment, dialysis of bacteriophage suspension is followed by the extraction of phage particles with chloroform:…

Melissa MartinezMarch 29, 2021

Laboratory Techniques Science

Electroporation-based CRISPR Cas9 delivery

CRISPR Cas9 is a potent and easy-to-use tool for gene editing, but the delivery of the CRISPR Cas9 system has been a challenge since it was developed. Several methods have been developed to effectively transport components of the CRISPR Cas9 system into the cells. The protocol below describes the electroporation-based…

Melissa MartinezMarch 29, 2021

Laboratory Techniques Protocols Science

Polyacrylamide Gel Electrophoresis

As an Amazon Associate, ConductScience Inc. earns revenue from qualifying purchases Polyacrylamide gel electrophoresis is one of the first forms of gel electrophoresis. Polyacrylamide is a synthetic polymer that is considered the most all-around separation matrix to date due to its transparency, electrical neutrality, and adjustable pore size. The technique…

Arpaporn SutipatanasomboonMarch 29, 2021

Laboratory Techniques Science

Purification of Bacteriophage λ Arms using Sucrose Density Gradients

Replacement vectors or substitution vectors are prepared by removing the central filler segment of phage DNA to accommodate the fragments of foreign DNA. This procedure is known as "preparation of λ arms." Restriction endonucleases are used to separate the two arms of phage DNA from the central stuffer fragment. Following…

Melissa MartinezMarch 29, 2021

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Biomolecules: Types and Functions

Capillary Electrophoresis

The Cryostat-Microtome

A Brief on The NMR Spectroscopy of Proteins

The Nuclear Overhauser Effect

Large Scale Extraction of Bacteriophage λ DNA Using Proteinase K

Electroporation-based CRISPR Cas9 delivery

Polyacrylamide Gel Electrophoresis

Purification of Bacteriophage λ Arms using Sucrose Density Gradients

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