Science - Conduct Science

Polyacrylamide Gel Electrophoresis

As an Amazon Associate, ConductScience Inc. earns revenue from qualifying purchases Polyacrylamide gel electrophoresis is one of the first forms of gel electrophoresis. Polyacrylamide is a synthetic polymer that is considered the most all-around separation matrix to date due to its transparency, electrical neutrality, and adjustable pore size. The technique…

ConductscienceMarch 29, 2021

Laboratory Techniques Science

Purification of Bacteriophage λ Arms using Sucrose Density Gradients

Replacement vectors or substitution vectors are prepared by removing the central filler segment of phage DNA to accommodate the fragments of foreign DNA. This procedure is known as "preparation of λ arms." Restriction endonucleases are used to separate the two arms of phage DNA from the central stuffer fragment. Following…

Melissa MartinezMarch 29, 2021

Laboratory Techniques Science

Preparation of Bacteriophage λ DNA Cleaved with two Restriction Enzymes to be used as a Cloning Vector

At each end of the central stuffer fragment, a series of restriction sites arranged in opposite directions are present in replacement vectors. For instance, in EMBL3A, the order of restriction site in the left polyclonal site is SalI-BamHI-EcoRI, and that of the right polyclonal site is EcoRI-BamHI-SalI. Digestion of this…

Melissa MartinezMarch 28, 2021

Laboratory Techniques Science

Alkaline Phosphatase Treatment of Bacteriophage λ Vector DNA

Alkaline phosphatase treatment is used to suppress the background of non-recombinants while performing restriction digestion with insertion vectors containing a single cloning site or polyclonal site using a single restriction enzyme. In this method, ligated DNA is first subjected to phenol: chloroform extraction and standard ethanol precipitation, respectively. It is…

Melissa MartinezMarch 26, 2021

Laboratory Techniques Protocols Science

Magnetic Resonance Imaging (MRI)

Magnetic resonance imaging is an advanced, non-invasive diagnostic medical imaging technique that provides three-dimensional images of organs. The discovery of MRI involves a series of successful events that started in 1946 when Felix Bolch proposed the new magnetic properties of the atomic nucleus. He explained the behavior of atoms at…

Anjali SinghMarch 15, 2021

Laboratory Techniques Protocols Science

Introduction to Preparative Electrophoresis

Electrophoresis techniques have been primarily developed for analytical purposes— to investigate the characteristics of the molecules in the sample of interest. The techniques can be modified to serve a preparative purpose, in which a large amount of a specific molecule can be obtained at the end of an electrophoresis run.…

ConductscienceMarch 8, 2021

Cart

The Nuclear Overhauser Effect

Large Scale Extraction of Bacteriophage λ DNA Using Proteinase K

Electroporation-based CRISPR Cas9 delivery

Polyacrylamide Gel Electrophoresis

Purification of Bacteriophage λ Arms using Sucrose Density Gradients

Preparation of Bacteriophage λ DNA Cleaved with two Restriction Enzymes to be used as a Cloning Vector

Alkaline Phosphatase Treatment of Bacteriophage λ Vector DNA

Magnetic Resonance Imaging (MRI)

Introduction to Preparative Electrophoresis

Our Company

For Scientists

OUR PRODUCTS