Techniques

Laboratory Techniques

Purification of Bacteriophage λ Arms using Sucrose Density Gradients

Introduction Replacement vectors or substitution vectors are prepared by removing the central filler segment of phage DNA to accommodate the fragments of foreign DNA. This procedure

Laboratory Techniques

Preparation of Bacteriophage λ DNA Cleaved with two Restriction Enzymes to be used as a Cloning Vector

Introduction At each end of the central stuffer fragment, a series of restriction sites arranged in opposite directions are present in replacement vectors. For instance, in

Laboratory Techniques

Alkaline Phosphatase Treatment of Bacteriophage λ Vector DNA

Introduction Alkaline phosphatase treatment is used to suppress the background of non-recombinants while performing restriction digestion with insertion vectors containing a single cloning site or polyclonal

Laboratory Techniques

Magnetic Resonance Imaging (MRI)

Introduction Magnetic resonance imaging is an advanced, non-invasive diagnostic medical imaging technique that provides three-dimensional images of organs. The discovery of MRI involves a series of

SDS-Polyacrylamide Gel Electrophoresis at Neutral pH (NuPAGE)

Laboratory Techniques

Introduction to Preparative Electrophoresis

Need Electrophoresis Equipment for your research? Click here Electrophoresis techniques have been primarily developed for analytical purposes— to investigate the characteristics of the molecules in the

Laboratory Techniques

The 𝛾-radiation and its effects on chromosomes

Introduction and Historical Perspectives Radiations have been extensively used in medical devices to look into the inner workings of the human body. Wilhelm Roentgen brought a

Laboratory Techniques

Drosophila: General Information and Methods for Experiments

Introduction Whoever thought that the small fly hovering over fruits will become such an essential asset for genetics research!! Fruit flies are one of the first

Laboratory Techniques

The Spiral Structure of Chromosome

Introduction A Chromosome is an organized package of DNA, located inside the nucleus. The structure of a chromosome involves six essential parts, that include: Pellicle and

Laboratory Techniques

Cellulose Acetate Electrophoresis

Need Electrophoresis Equipment for your research? Click here Cellulose acetate electrophoresis is a type of zone electrophoresis that separates molecules in liquid form such as proteins

Laboratory Techniques

The Cytogenetics of Fungi

As an Amazon Associate, ConductScience Inc. earns revenue from qualifying purchases Introduction Fungi is a group of eukaryotic organisms that include a myriad of single and

Laboratory Techniques

Human Sex Chromosomes and X-inactivation

Introduction Chromosomes are thread-like structures, located in the nucleus, which carry hereditary information in the form of genes. Structurally, it is composed of ‘packaging proteins and

Laboratory Techniques

Estimating the Chiasma Frequency in Diplotene-Diakinesis Stage

Introduction Chiasma is the point of crossing over or site where the exchange of genetic material takes place between two homologous, non-sister chromatids. The crossover occurs