Techniques
Large Scale Extraction of Bacteriophage λ DNA Using Proteinase K
Introduction and Principles Large-scale extraction of phage DNA involves digestion of viral coat proteins using a strong protease such as proteinase K followed by extraction with
Electroporation-based CRISPR Cas9 delivery
CRISPR Cas9 is a potent and easy-to-use tool for gene editing, but the delivery of the CRISPR Cas9 system has been a challenge since it was
Polyacrylamide Gel Electrophoresis
As an Amazon Associate, ConductScience Inc. earns revenue from qualifying purchases Polyacrylamide gel electrophoresis is one of the first forms of gel electrophoresis. Polyacrylamide is a
Purification of Bacteriophage λ Arms using Sucrose Density Gradients
Introduction Replacement vectors or substitution vectors are prepared by removing the central filler segment of phage DNA to accommodate the fragments of foreign DNA. This procedure
Preparation of Bacteriophage λ DNA Cleaved with two Restriction Enzymes to be used as a Cloning Vector
Introduction At each end of the central stuffer fragment, a series of restriction sites arranged in opposite directions are present in replacement vectors. For instance, in
Alkaline Phosphatase Treatment of Bacteriophage λ Vector DNA
Introduction Alkaline phosphatase treatment is used to suppress the background of non-recombinants while performing restriction digestion with insertion vectors containing a single cloning site or polyclonal
Magnetic Resonance Imaging (MRI)
Introduction Magnetic resonance imaging is an advanced, non-invasive diagnostic medical imaging technique that provides three-dimensional images of organs. The discovery of MRI involves a series of
Introduction to Preparative Electrophoresis
Need Electrophoresis Equipment for your research? Click here Electrophoresis techniques have been primarily developed for analytical purposes— to investigate the characteristics of the molecules in the
The 𝛾-radiation and its effects on chromosomes
Introduction and Historical Perspectives Radiations have been extensively used in medical devices to look into the inner workings of the human body. Wilhelm Roentgen brought a
Drosophila: General Information and Methods for Experiments
Introduction Whoever thought that the small fly hovering over fruits will become such an essential asset for genetics research!! Fruit flies are one of the first
The Spiral Structure of Chromosome
Introduction A Chromosome is an organized package of DNA, located inside the nucleus. The structure of a chromosome involves six essential parts, that include: Pellicle and
Cellulose Acetate Electrophoresis
Need Electrophoresis Equipment for your research? Click here Cellulose acetate electrophoresis is a type of zone electrophoresis that separates molecules in liquid form such as proteins
