Techniques
Protein Staining Methods
Introduction Protein separation and identification is critical for proteome analysis and requires high resolution and powerful protein characterization after gel electrophoresis. To identify the proteins in
Zinc Staining Protocol
Introduction The reversible Zinc stain is a widely used staining method for the detection of proteins fractionated by polyacrylamide gels or SDS denatured gels. The Zinc
Ponceau S Stain Protocol
Need stains for your experiments? Click here Introduction Ponceau S staining is a rapid and reversible staining method used for the detection of protein bands on
Pulse Chase Analysis
Need Incubators for your lab? Click here  As an Amazon Associate Conductscience Inc earns revenue from qualifying purchases  Introduction The pulse-chase analysis is a
Silver Staining Protocol
Need stains for your experiments? Click here Silver staining is a powerful technique for protein identification in gels as silver binds to chemical sidechains of the
Southern Blotting Protocol
Need Laboratory Equipment for your reasearch? Click here Southern blotting is a hybridization technique used for deoxyribose nucleic acid (DNA). The method was named after Edward
Affinity Chromatography Protocol
Introduction Affinity chromatography is a versatile separation protocol that uses the biological interactions for characterization and detailed analysis of sample components. It is based on highly
BRADFORD Protein Determination Protocol
Need Laboratory Equipment for your reasearch? Click here Introduction and Principle Developed by Marion M. Bradford in 1976, the Bradford assay is a quick protocol, and
Fast protein liquid chromatography (FPLC) Protocol
Introduction Fast protein liquid chromatography (FPLC) is a type of liquid chromatography that provides high resolution by small-diameter stationary phases for protein characterization and separation. The
Gel Filtration Chromatography Protocol
Need benchtop equipment for your lab? Click here Introduction Gel-filtration chromatography, also known as size exclusion chromatography, is a versatile technique that permits the separation of
Ion-exchange Chromatography Protocol
Introduction Ion Exchange Chromatography (IEC) is a powerful liquid chromatographic technique used for bioseparation. The separation is done by a reversible interaction between charged molecules of
High performance liquid chromatography (HPLC) Protocol
Introduction High-performance liquid chromatography (HPLC) is a powerful separation technique used for the analysis of ions, proteins, and organic molecules. HPLC is based on the mechanisms
