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Laboratory TechniquesProtocols
June 26, 2019

SDS-Polyacrylamide Gel Electrophoresis at Neutral pH (NuPAGE)

The traditional Laemmli system denaturing conditions are stimulated by the Invitrogen NuPAGE SDS-PAGE gel system which is a revolutionary high-performance polyacrylamide gel electrophoresis system. NuPAGE gels use a unique buffer formulation to maintain a neutral operating pH during electrophoresis, which minimizes protein modifications that can result in poor band resolution.…
Nucleic Acid Quantification Protocols
Laboratory TechniquesProtocols
June 25, 2019

Nucleic Acid Quantification Protocols

DNA and RNA quantifications are broadly utilized in biological and biomedical research. Over the last ten years, many technologies have been developed to enable automated and high-throughput analyses. We are going to learn the assays and different methods of how DNA and RNA quantifications are carried out.This procedure has been…
Filtration Chromatography Protocol
Laboratory TechniquesProtocols
June 25, 2019

Quantitative Phosphate Protocol

To find out the inorganic phosphate in a protein-containing liquid biological sample an aqueous reagent comprises an acid. This acid is capable of reacting with the molybdate salt to form molybdic acid for complexation with phosphate to form phosphomolybdate complexes, a molybdate salt, a nonionic surfactant reagent in an amount…
Laboratory TechniquesProtocols
June 25, 2019

TRICINE-SDS-PAGE Protocol

Proteins in the mass range 1–100 kDa are commonly separated by Tricine–SDS-PAGE. For the resolution of proteins less than 30 kDa, it is the preferred electrophoretic system. As compared to other electrophoretic systems, the concentrations of acrylamide used in the gels are lower. Electroblotting is facilitated by these lower concentrations,…
Laboratory TechniquesProtocols
June 23, 2019

Western blotting Protocol

The Western blot is a biological technique that allows for specific identification and characterization of proteins. In this technique, the proteins are assorted based on their molecular weight, and type, through gel electrophoresis. The results of the electrophoresis are then transferred to a polyvinylidene fluoride (PVDF) membrane producing a band…
Electromyography
Laboratory TechniquesScience
April 26, 2019

Electromyography

Introduction Electromyography (EMG) is a specialized diagnostic technique used to study the muscle function by analyzing the electrical signals emanating from the nerves and the muscles. EMG can distinguish the abnormal or diseased muscle from the normal muscle. The electromyography can detect muscular abnormalities such as fasciculation or chronic denervation…
Safranin Staining By Conductscience
Laboratory TechniquesProtocolsScience
April 25, 2019

Safranin Staining

Introduction Safranin is a cationic dye used in histology and cytology to distinguish and identify different tissues and cells.  It is popular in medical research for staining acidic proteoglycan that is found in cartilage tissues, enabling the researchers to analyze cell chondrogenesis. The safranin is employed as a counter-stain in…
Laboratory TechniquesProtocolsScience
June 8, 2018

Animal Identification Techniques

Scientific investigations, preclinical research, and pharmacological studies use a number of laboratory animals as subjects. Therefore, proper animal identification becomes the necessity. Animal identification techniques not only help keep a record of the animals but also aids communication between the healthcare professionals and researchers. Furthermore, the identification systems help experimenters…