Search
Generic filters
All
Products
Blogs

Site-directed mutagenesis

Enzymology
Enzymology

Site-Directed Mutagenesis: Methods and Applications

Site-directed mutagenesis is a technique conceived in the 70s used to mutate specific DNA sequences in vitro. It relies on synthetic short single-stranded DNA fragments, or oligonucleotides, that contain designated mutations to act as templates in the presence of DNA polymerase enzyme.[1] In the late 80s, Polymerase Chain Reaction (PCR), another laboratory technique, was incorporated into site-directed mutagenesis workflow. The