spectroscopy

Laboratory Techniques

A Brief on The NMR Spectroscopy of Proteins

Spectroscopy is “the study of investigation and measurement of the absorption and emission of light or electromagnetic radiation by matter as a function of its wavelength and frequency”. In simple terms, it’s the study of the interaction of light with a matter or substance. Depending on the nature of the interaction between the energy and material, spectroscopy is divided into

April 13, 2021

Laboratory Techniques

The Nuclear Overhauser Effect

Introduction The nuclear overhauser effect is defined as a change in the integrated intensity of one spin when the equilibrium population of another spin is perturbed by saturation or inversion. So, it is the transfer of nuclear spin polarization from one spin-active nucleus to another. However, the nuclei being irradiated should be closer to the other nuclei, in space, to

April 5, 2021

Laboratory Techniques

Biomolecular NMR Spectroscopy

The information about the structure, interactions, and dynamics of biomolecules (protein, DNA, and RNA) is essential for understanding cellular processes. This knowledge is deconvolved by scientists and used in elucidating cellular mechanisms for finding cures for human diseases. The pharmaceutical industry, for example, uses this structural information to improve on the already discovered drugs or to find new ones. There

April 13, 2020

Lab Basics

Fluorescence Spectrophotometry: Principles and Applications

Fluorescence spectrophotometry is a set of techniques that deals with the measurement of fluorescence emitted by substances when exposed to ultraviolet, visible, or other electromagnetic radiation. It has wide application in chemical and biological sciences as it can be used to analyze a biological system, by studying its interactions with fluorescent probe molecules (So and Dong, 2002, The International Pharmacopoeia,

November 28, 2019

Lab Basics

Nuclear Magnetic Resonance Spectroscopy

Two molecules may have the same number and type of atoms, but their properties would change depending on how they are arranged (i.e., the bonds linking them and their orientation). As an example, Ethanol and Dimethyl ether, both have one oxygen, two carbon, and six hydrogen atoms, but the structures and properties of both these compounds are different. Ethanol exists

November 21, 2019

Lab Basics

The Basics of Infrared Spectrophotometry

Spectroscopy refers to the interaction between electromagnetic (EM) radiation and matter as a function of its frequency or wavelength. From this, a number of quantitative and qualitative measurements defining aspects like sample concentrations, structure, chemical compositions etc, can be obtained depending on the spectroscopic technique and spectrum of EM radiation used. When EM radiation interacts with matter, its frequency can

October 10, 2019

A Brief on The NMR Spectroscopy of Proteins

The Nuclear Overhauser Effect

Biomolecular NMR Spectroscopy

Fluorescence Spectrophotometry: Principles and Applications

Nuclear Magnetic Resonance Spectroscopy

The Basics of Infrared Spectrophotometry

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