Calculate split ratios, seeding densities, and passage schedules for adherent and suspension cell lines. Growth curve prediction with doubling time modeling. Data never leaves your browser.
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Load example cell passaging data to see the full workflow
| Passage | Time | When |
|---|---|---|
| P1 | 48h | Day 2, ~0h |
| P2 | 96h | Day 4, ~0h |
| P3 | 144h | Day 6, ~0h |
| P4 | 192h | Day 8, ~0h |
| P5 | 240h | Day 10, ~0h |
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The split ratio describes the dilution factor at passage. A 1:4 split means seeding one-quarter of the harvested cells into each new vessel.
Split ratio = (cells harvested) / (cells seeded per vessel)
Higher ratios (1:10, 1:20) are economical but delay confluence. Lower ratios (1:2, 1:3) maintain faster growth but require more frequent passages. Most standard protocols use 1:3 to 1:6 for routine maintenance.
Cells in log-phase follow exponential growth:
N(t) = N₀ 2^(t/Td)
Where N₀ is the initial count, t is time in hours, and Td is the doubling time. This model applies during log-phase growth (approximately 20-80% confluence). At very low or very high densities, growth deviates due to lag phase and contact inhibition, respectively.
