Drosophila Locomotion Video Checklist
Generate a printable filming protocol with camera positions, locomotion types, apparatus, acclimation notes, and standardized file naming for Drosophila gait and locomotion studies or ConductVision upload.
Try it out
Load example Drosophila locomotion video checklist data to see the full workflow
Session Info
Locomotion Types
Camera Positions
Apparatus
Filming Protocol (0 clips)
File naming: _adult_2026-04-29_[LOCO]_[CAMERA]_[APPARATUS]_trial[N]
Select locomotion types, camera positions, and apparatus to generate your protocol.
Analyze your Drosophila locomotion videos with ConductVision
Upload your standardized clips for AI-powered locomotion analysis, speed quantification, and behavioral scoring.
Go to ConductVisionFilming protocol follows established Drosophila locomotion methodologies (RING assay, DAM system, FlyTracker). Upload completed videos to ConductVision for AI locomotion analysis.
How It Works
Select the developmental stage (adult or larva), locomotion types (walking, climbing, flight, startle response, courtship walk, larval crawling), camera positions (dorsal, lateral, ventral, high-mag), and apparatus (climbing vial, open arena, Y-maze, DAM tube, flight column, agar plate). Optionally enable high-speed video notes, acclimation protocols, and group size recommendations. The tool generates a numbered shot list with Drosophila-specific instructions and duration for each clip. Incompatible combinations (e.g. larval crawling with climbing vial) are automatically filtered. Download the protocol as a printable PDF.
Filming Best Practices
CO2 anesthesia requires minimum 1 hour recovery before locomotor testing — this is the most common source of artifact. Age-standardize your flies (3–5 day old adults for most assays). Separate males and females as they have different locomotor profiles. Maintain 25°C ± 1°C during recording. Test at consistent time in the light cycle. For negative geotaxis, use 10-sec recording after gentle tap to bottom, with 2–3 practice trials. For larval crawling, use dark agar (2% agarose) for contrast. Frame rate: 30 fps for walking speed, 100+ fps for leg coordination. Drosophila are extremely vibration-sensitive — isolate the recording setup.