ToolsConductScience tool
100% Client-SideFree in-browser calculator

FCS Validator.

Validate and repair FCS 2.0/3.0/3.1/3.2 flow cytometry files. Fix noncompliant headers and offsets, preview events, export to CSV.

PrivateData stays in your browser
LiveNo sign-up required
Validated2026-05-24
CitableMethods and citation included

Calculator

Results update in place

No file handy? (synthetic 4-channel, 500 events)

Format Details

Extensions
.fcs
Domain
Immunology / Cell Biology
Legacy software
FlowJo, FCS Express
License
NIST Fireflow (public-service license)

Privacy

  • Files processed in your browser
  • No data uploaded to servers
  • No account required
  • Research use only
1

Method

Uses the tool-specific formulas and assumptions shown in the calculator output.

2

Validated

Last validated 2026-05-24. Calculations are designed for planning and documentation support; verify procurement decisions against manufacturer specifications or institutional SOPs.

3

How to cite

ConductScience. ConductScience tool (v1.0.0). 2026.

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