Social Interaction Analyzer

Score direct social investigation, resident-intruder aggression rate, group SEM, and CSV export for rodent social behavior studies.

Resident-IntruderDyadicCSV Export

Social interaction scoring table

Enter event durations from resident-intruder or freely interacting dyads. Social investigation is the sum of nose-to-nose, anogenital sniffing, and allogrooming time divided by session duration.

AnimalGroupSession (s)Nose-nose (s)Anogenital (s)Allogroom (s)Aggression boutsSocial %Aggression/minRemove
24.0%0.10
22.8%0.00
11.7%0.50
10.5%0.60
AnimalGroupSocial investigation %Aggression/min
WT-1WT24.000.10
WT-2WT22.830.00
KO-1KO11.670.50
KO-2KO10.500.60

Group social investigation

Group aggression rate

  • Compute social-investigation percent from direct interaction scoring
  • Compare resident-intruder aggression rates across groups
  • Summarize genotype or treatment cohorts with mean plus SEM
  • Export animal-level event totals for Prism, R, or Python
  • Pair direct-contact assays with three-chamber sociability data

Don't use for

  • Three-chamber chamber-time or social novelty preference indices
  • Tube dominance scoring
  • Automated pose-estimation workflows that already export complete ethograms

Resources

  • Resident-intruder or dyadic mode selected before scoring
  • Session duration fixed within each cohort
  • Partner novelty and sex pairing documented
  • Nose-to-nose, anogenital, and allogroom definitions written down
  • Aggression criteria defined for resident-intruder sessions
  • Scorer blinded to group when possible

Direct Social Interaction Assays

Direct social interaction assays measure how much time a test animal spends investigating another animal. Resident-intruder designs emphasize territorial and aggressive behavior. Dyadic designs emphasize reciprocal interaction in a neutral or familiar arena.

Metrics and Math

The analyzer sums nose-to-nose, anogenital sniffing, and allogrooming durations for each animal or dyad. That total is divided by session duration to report social-investigation percent. In resident-intruder mode, aggression bouts are divided by session minutes.

Design Notes

Keep lighting, arena, partner novelty, resident isolation period, and scoring definitions constant across groups. Blind manual scorers to genotype or treatment when feasible. Pair with video scoring when behaviors are brief or overlapping.

Frequently Asked Questions