Wire Hang Test Calculator

Calculate best-of-three and mean hang latency with cutoff clamping, group SEM, and CSV export for neuromuscular phenotyping.

Best HangMean HangCSV Export

Wire hang latency table

Enter fall latency for each hang trial. The default protocol uses 3 trials, 180 second cutoff, and 60 second inter-trial interval.

Animal IDGroupT1 (s)T2 (s)T3 (s)

Cutoff: 180s. Values exceeding cutoff are clamped on calculation.

Animal summary

AnimalGroupBest hang (s)Mean hang (s)Cutoff
M1WT180.00131.67clamped
M2SMA42.0036.00none

Group best hang time

Group mean hang time

  • Compute best-of-three and mean wire hang latency
  • Apply a prespecified cutoff before best and mean are calculated
  • Compare neuromuscular phenotype groups with mean plus SEM
  • Export animal-level clamped hang data for Prism, R, or Python
  • Pair wire hang with rotarod, pole, beam walk, and cylinder endpoints

Don't use for

  • Peak pull-force grip strength meter analysis
  • Detailed gait kinematics or paw-placement scoring
  • Forced exercise endurance protocols such as treadmill running

Resources

  • Wire or grid variant documented
  • Wire diameter and height recorded
  • Landing padding in place
  • Cutoff selected before testing
  • Inter-trial interval fixed across animals
  • Body weight recorded near test date

What Is the Wire Hang Test?

The wire hang test is a simple neuromuscular endurance assay. A rodent is placed on a wire or grid, inverted or suspended according to the protocol, and the observer records latency to fall.

The endpoint is useful in ALS, SMA, muscular dystrophy, aging, and pharmacology studies where endurance and grip maintenance are expected to change.

Metrics and Math

This calculator clamps every entered trial at the selected cutoff. It reports best hang time as the maximum clamped trial for each animal and mean hang time as the average clamped trial value.

Group summaries are computed from animal-level best or mean values and reported as mean plus SEM.

Best Practices

Keep wire diameter, test height, landing padding, handling method, cutoff, and inter-trial interval consistent. Exclude or flag animals with paw injury or handling events according to prespecified criteria.

Because body weight affects the physical load, record weight near the test date and consider it during interpretation.

Frequently Asked Questions